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Forum: RNA Sequencing 07-13-2018, 11:28 AM
Replies: 1
Views: 977
Posted By Zero/1
single-cell cDNA left 90 min at 95C

Briefly, cDNA from single neurons prepared using SMART-Seq4 kit spent 90 minutes at 95C due to human error. Is the cDNA to be discarded or can it still be used for library preparation with Nextera...
Forum: Sample Prep / Library Generation 06-15-2018, 09:14 PM
Replies: 3
Views: 953
Posted By Zero/1
How to resuspend Ampure beads which fail to resuspend?

I drammatically overdried my Ampure beads, now even pipetting over and over I cannot resuspend them: the lie at the bottom of the tube in large clumps. Any idea? I am afraid I cannot mess this...
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