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Forum: Sanger/Dye Terminator 04-16-2019, 07:04 AM
Replies: 1
Views: 4,443
Posted By beetle_doc
Both work well.

Both work well.
Forum: Illumina/Solexa 11-08-2016, 12:27 PM
Replies: 2
Views: 1,152
Posted By beetle_doc
custom sequencing primers on HiSeq3000

I know it is not supported but has anyone tried to use custom sequencing primers on the HiSeq3000/4000?
Thanks
Forum: Illumina/Solexa 08-25-2015, 06:28 AM
Replies: 2
Views: 1,583
Posted By beetle_doc
Thanks for the response. I will pass the...

Thanks for the response. I will pass the information along to my client.
Mike
Forum: Illumina/Solexa 08-21-2015, 08:50 AM
Replies: 2
Views: 1,583
Posted By beetle_doc
Odd bioanalyzer results

I had a customer that submitted some antibody libraries which I ran on our MiSeq. The results looked good up until about 100 bases then the Q30 took a nose dive and the %A increased, suggesting that...
Forum: Illumina/Solexa 02-13-2015, 12:39 PM
Replies: 2
Views: 1,205
Posted By beetle_doc
Keeping business from going elsewhere

Don't know if this is the right forum for this question. We have lost a substantial amount of our on campus illumina sequencing business to other service providers (mostly BGI) over the last year. ...
Forum: Illumina/Solexa 06-27-2014, 08:45 AM
Replies: 2
Views: 1,408
Posted By beetle_doc
Constant issues with the HiSeq 2500

We have had a HiSeq 2000/2500 since 2011 that breaks down quite frequently. This round of issues has been going on since around February. It started with no intensity on read 2 of high output runs...
Forum: Core Facilities 03-03-2014, 12:58 PM
Replies: 1
Views: 10,512
Posted By beetle_doc
genotyping by sequencing as service

I have a meeting coming up to discuss the possibility of our core lab offering genotyping by sequencing as a service. I was wondering if anyone who currently offers this service has any advice that...
Forum: Sample Prep / Library Generation 04-16-2012, 07:53 AM
Replies: 2
Views: 3,128
Posted By beetle_doc
That sounds great. What version of Pippin prep...

That sounds great. What version of Pippin prep are you using?
Mike
Forum: Sample Prep / Library Generation 04-05-2012, 11:25 AM
Replies: 2
Views: 3,128
Posted By beetle_doc
Pippin Prep for TruSeq small RNA libraries

Is anyone using the Pippin Prep to size select their TruSeq small RNA libraries? If so, could you give me information on ease of use, failure rate, and % primer dimer sequences per sample. We are...
Forum: Sample Prep / Library Generation 03-07-2012, 10:41 AM
Replies: 1
Views: 3,770
Posted By beetle_doc
strange library results

I have run 3 sample using TruSeq genomic prep using both gel and non gel based methods. The resulting libraries when run on a bioanalyzer all looked like lane 6 of the attached document. Other...
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