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Forum: Illumina/Solexa 08-12-2019, 10:43 AM
Replies: 3
Views: 431
Posted By ChiaraGen
Thank you very much for your help! What about...

Thank you very much for your help!
What about Q2, I have found a paper
https://www.nature.com/articles/s41598-017-03448-8

where the author writes:

"DNA exists naturally as a double-stranded...
Forum: Illumina/Solexa 08-11-2019, 12:08 AM
Replies: 3
Views: 431
Posted By ChiaraGen
NGS sequencing and capture probes

Hello!

I have two questions for you:

1.I don't understand,when a library is denatured before loading in the ngs instrument, I'll have the two complementary strands separately; so they will form...
Forum: Illumina/Solexa 08-01-2019, 06:07 AM
Replies: 0
Views: 407
Posted By ChiaraGen
Karyomapping Illumina

Hello!

Someone Knows how does it works Karyomapping by Illumina? I know that by SNPs array analysis it permits to reconstruct haplotypes and to knows if a particular mutation is transmitted to...
Forum: Bioinformatics 07-31-2019, 12:05 AM
Replies: 0
Views: 398
Posted By ChiaraGen
Off-target

Hello,
I have some questions about off-target. If I have understand well off-target can rise from probes that have promiscuous hybridization and recognize more sites in the genome, but also it can...
Forum: Illumina/Solexa 07-11-2019, 04:36 PM
Replies: 0
Views: 563
Posted By ChiaraGen
Pcr bridge error rate and duplicates

Hello!
I have 3 questions,can you help me please to understand?
1. Why almost only the pcr step in the library prep is cited as source of errors while bridge amplification not?
I have found only...
Forum: Sample Prep / Library Generation 03-26-2019, 04:23 AM
Replies: 0
Views: 463
Posted By ChiaraGen
Haloplex HS method

Hello!
I have two questions about Haloplex HS protocol:

1. I read that Haloplex uses double stranded byotinilated probes and during hybridization step I put with probes also indexes. Someone...
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