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Forum: Sample Prep / Library Generation 12-24-2009, 06:37 AM
Replies: 1
Views: 2,454
Posted By floor121
If you use the illumina kit to construct library,...

If you use the illumina kit to construct library, 15 min is enough for adaptor ligation; if not, the ligation time should be optimized.
Forum: Illumina/Solexa 09-26-2009, 05:36 PM
Replies: 6
Views: 2,723
Posted By floor121
Maybe you could reduce the cluster density, 21k...

Maybe you could reduce the cluster density, 21k seems a little high...
Forum: Illumina/Solexa 09-22-2009, 05:41 PM
Replies: 6
Views: 2,723
Posted By floor121
Only the miRNA library have lot of 'N" in this...

Only the miRNA library have lot of 'N" in this run? How about the cluster density?
Forum: Illumina/Solexa 01-18-2009, 10:57 PM
Replies: 11
Views: 4,760
Posted By floor121
I try to sample prep with the index kit last...

I try to sample prep with the index kit last month, it seems work well and the workflow is almost the same as PE library prep except PCR, however, the index libraries cannot run with normal PE...
Forum: Illumina/Solexa 10-22-2008, 05:32 PM
Replies: 13
Views: 3,860
Posted By floor121
Thans ECO. Actually I fragment our sample...

Thans ECO.
Actually I fragment our sample following the protocol from SOLiD, and no glycerol has been used. So we can measure the concentraion before clean up.
I clean up our sample using Qiagen...
Forum: Illumina/Solexa 10-22-2008, 02:40 AM
Replies: 13
Views: 3,860
Posted By floor121
Covaris recovery ratio

Hi,
Recently I am try to fragment genomic DNA by Covaris, and found something interesting. After DNA fragment I measure the concentration by nanodrop and Agilent 2100, the concentration from Agilent...
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