Forum: Illumina/Solexa
12-17-2012, 08:21 AM
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Replies: 3
Views: 1,188
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Forum: Illumina/Solexa
12-14-2012, 10:42 AM
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Replies: 3
Views: 1,188
Locating file in SRA
I wanted to locate some illumina sequencing data for humans of size 130bp and 150bp from the SRA. However, searching with keywords like "Illumina" "130bp" "Human" etc. does not give any results or...
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Forum: Illumina/Solexa
04-07-2012, 01:35 PM
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Replies: 2
Views: 1,598
Thanks a lot twaddlac, I have solved my problem...
Thanks a lot twaddlac, I have solved my problem using the SRA toolkit.
For splitting SRA fastq to .qual and .csfasta I used the abi-dump tool in SRA toolkit. The script solid2fastq.pl in BWA fails...
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Forum: Illumina/Solexa
04-06-2012, 12:25 PM
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Replies: 2
Views: 1,598
SOLiD to NCBI SRA
Hi,
Can anyone help me to convert SOLiD data in .qual and .csfasta format into the FASTQ format used by NCBI SRA? Similaly, is there a way to separate a color space FASTQ in NCBI SRA into...
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Forum: Bioinformatics
01-11-2012, 11:03 AM
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Replies: 0
Views: 1,350
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Forum: Bioinformatics
12-10-2010, 12:26 AM
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Replies: 0
Views: 1,473
BWA:Unique reads in seeding mode
Hi,
When seeding is enabled in BWA, if a read is marked as unique does it mean that the seed portion is uniquely mapped, like in ELAND? or is it that the whole read is uniquely mapped?
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Forum: Bioinformatics
12-04-2010, 02:27 AM
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Replies: 3
Views: 1,994
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Forum: Bioinformatics
12-03-2010, 11:53 PM
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Replies: 3
Views: 1,994
How to map NCBI SOLiD reads with BWA
Hi,
I have downloaded some SOLiD reads from NCBI and needs to map them using BWA. Should I simply convert 0->A, 1->C etc. or do I need to chop the first two bases in addition?
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