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Forum: Literature Watch

04-01-2016, 05:14 AM

Replies: 2

RNA-Seq: RASL-seq for Massively Parallel and Quantitative Analysis of Gene Expression

Views: 7,590

Posted By cvhove

RASL-seq

anybody got this working? ( year later?)

Forum: Illumina/Solexa

10-08-2013, 01:20 AM

Replies: 5

Magnetic bead washers

Views: 2,823

Posted By cvhove

hoi benny, we have adapted a bead...

hoi benny,

we have adapted a bead purification protocol on the Biomek 3000 platform and have to admit that this works well.

Forum: Sample Prep / Library Generation

06-26-2012, 12:47 AM

Replies: 5

RNA-seq: rRNA Depletion, Double riboZero or Double RiboMinus

Views: 4,914

Posted By cvhove

RNA-seq: rRNA Depletion, Double riboZero or Double RiboMinus

Hi,
I did a RNA-seq sample on Illumina GA2x: 15 years old Tumor FFPE sample. The RNA extraction give a very low RIN score analysing on the BioAnalyser RIN +/-1.5.
I used the EPICENTRE combination...

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