Forum: Academic/Non-Profit Jobs
05-17-2015, 01:12 PM
|
|
Replies: 0
Views: 1,489
|
|
Forum: Academic/Non-Profit Jobs
12-16-2013, 03:09 PM
|
|
Replies: 1
Views: 5,469
|
|
Forum: Sample Prep / Library Generation
12-02-2013, 09:22 AM
|
|
Replies: 0
Views: 1,682
|
|
Forum: Ion Torrent
12-02-2013, 09:16 AM
|
|
Replies: 9
Views: 6,481
|
|
Forum: Ion Torrent
11-25-2013, 09:29 AM
|
|
Replies: 2
Views: 3,412
|
|
Forum: Ion Torrent
11-12-2013, 05:21 AM
|
|
Replies: 14
Views: 7,570
We are using the RNAseq v2 kit routinely for...
We are using the RNAseq v2 kit routinely for viral RNA sequencing, miRNA sequencing, and polyA+ libraries too. Works quite well. We did have a Ashe of a bad lot / kit once and life tech replaced the...
|
|
Forum: Academic/Non-Profit Jobs
09-30-2013, 10:28 AM
|
|
Replies: 1
Views: 5,469
|
|
Forum: Ion Torrent
09-24-2013, 09:54 AM
|
|
Replies: 8
Views: 11,194
Also - keep in mind that Q30 Ion data != Q30...
Also - keep in mind that Q30 Ion data != Q30 Illumina data since the base calling algorithms are different and the estimation/prediction of "quality" is inherently different between the base calling...
|
|
Forum: Ion Torrent
08-26-2013, 05:00 AM
|
|
Replies: 1
Views: 4,720
|
|
Forum: Ion Torrent
07-15-2013, 05:22 AM
|
|
Replies: 2
Views: 4,158
|
|
Forum: Ion Torrent
07-01-2013, 04:19 AM
|
|
Replies: 7
Views: 6,503
For what it's worth - higher coverage will NOT...
For what it's worth - higher coverage will NOT not lead to better de novo assemblies. Once you hit about 40-50x , higher coverage may actually produce worse assemblies. Using an Ion318 for a 2MB...
|
|
Forum: Ion Torrent
05-13-2013, 05:26 PM
|
|
Replies: 2
Views: 5,234
The PGM doesn't have a 10G chip.
Do you...
The PGM doesn't have a 10G chip.
Do you mean the Proton system? That will get you about 100M reads at 200bp; should take a couple of hours on the hardware that ships with the Proton (if you use...
|
|
Forum: Ion Torrent
05-13-2013, 05:25 PM
|
|
Replies: 1
Views: 3,235
For single read 200bp data - In our experience...
For single read 200bp data - In our experience with the PGM running 200bp reads - that's about right. You'll be lucky if you break 100K for your largest contig for most bacterial genomes with single...
|
|
Forum: Ion Torrent
05-13-2013, 05:21 PM
|
|
Replies: 3
Views: 3,352
|
|
Forum: Ion Torrent
02-07-2013, 04:51 AM
|
|
Replies: 9
Views: 3,740
|
|
Forum: Bioinformatics
02-06-2013, 09:21 AM
|
|
Replies: 2
Views: 1,539
Are you using GW6?
Tracks is not the way to...
Are you using GW6?
Tracks is not the way to do this - you should use a Read Mapping file; not a Track file.
This might be a hack - but you might try annotating the genome with a custom...
|
|
Forum: Ion Torrent
08-14-2012, 12:38 PM
|
|
Replies: 4
Views: 3,195
A couple suggestions that might help:
First...
A couple suggestions that might help:
First - depending on your application, you may also want to look into a viral quasispecies analysis tool such as ShoRAH or QuRe - the viral quasispecies...
|
|
Forum: Ion Torrent
07-30-2012, 01:31 PM
|
|
Replies: 1
Views: 3,305
|
|
Forum: Bioinformatics
07-11-2012, 10:03 AM
|
|
Replies: 12
Views: 21,936
|
|
Forum: Ion Torrent
07-09-2012, 07:59 AM
|
|
Replies: 27
Views: 19,265
|
|
Forum: Ion Torrent
04-09-2012, 04:03 AM
|
|
Replies: 18
Views: 10,508
It actually an either/or question - not both.
...
It actually an either/or question - not both.
The idea behind using the BioAnalyzer is that you can assess your library's quality far far better than using a gel. When we first started running the...
|
|
Forum: Ion Torrent
03-21-2012, 06:43 AM
|
|
Replies: 15
Views: 14,886
|
|
Forum: Bioinformatics
02-28-2012, 10:01 AM
|
|
Replies: 8
Views: 11,136
BUMPED.
Thank you for this. We're using CLC...
BUMPED.
Thank you for this. We're using CLC Bio for routine analysis (which is really nice) but it doesn't support circular genome maps at the moment.
So far BRIG has done what I need. CIRCOS...
|
