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Forum: General 06-04-2013, 06:41 AM
Replies: 1
Views: 1,346
Posted By Nullie
16s cDNA

Is it possible to turn 16s rRNA into cDNA?
or is cDNA only synthesized by mRNA?
Forum: RNA Sequencing 06-03-2013, 01:07 PM
Replies: 10
Views: 3,013
Posted By Nullie
sequencing 16s rRNA

Is it possible to sequence the 16s rRNA of bacteria using RNA-SEQ?

If not, how can I sequence it and use that to check for different bacteria ditribution in my sample?
Forum: Bioinformatics 06-03-2013, 01:02 PM
Replies: 1
Views: 568
Posted By Nullie
checking for bacterial distribution

how can I check the bacteria distribution in a human gut?

If I extract a DNA sample and isolate all bacterial DNA from it can I then sequence all 16s rDNA's and somehow check the expression level...
Forum: Illumina/Solexa 06-03-2013, 12:38 PM
Replies: 7
Views: 1,386
Posted By Nullie
is it possible to isolate 16srRNA from the total...

is it possible to isolate 16srRNA from the total RNA sample?
Forum: Illumina/Solexa 06-03-2013, 12:02 PM
Replies: 5
Views: 934
Posted By Nullie
If I dont have a reference genome to map against...

If I dont have a reference genome to map against which should I use?
Forum: Illumina/Solexa 06-03-2013, 09:59 AM
Replies: 5
Views: 934
Posted By Nullie
thank you

Are there more advantages to PE over SE?
Forum: Illumina/Solexa 06-03-2013, 03:01 AM
Replies: 5
Views: 934
Posted By Nullie
mapping advantages

Is there an advantage to mapping using single end reads over paired end reads? or the other way around?
will there be a difference in the accuracy of the mapping between the two options?

Thank...
Forum: Illumina/Solexa 06-02-2013, 05:06 AM
Replies: 7
Views: 1,386
Posted By Nullie
Isolating microbial RNA

I want to do check the microbial population in the gut before and after treatment, I isolated the total RNA from the intestine but how can I isolate the bacterial RNA from human RNA?
and after...
Forum: Illumina/Solexa 06-02-2013, 12:53 AM
Replies: 2
Views: 1,105
Posted By Nullie
Help to plan experiment

Hello everyone,

I am a biology student and I need to check the microbial population in the intestine before and after taking a certain medication, to see if the drug affects the microbe abundance...
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