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Forum: Sample Prep / Library Generation 12-07-2008, 11:16 PM
Replies: 3
Views: 3,555
Posted By fedora
Thanks cjohns. We dont see any 80bp or 120b...

Thanks cjohns. We dont see any 80bp or 120b products in these libraries (our DNA is 150-300bp) but maybe there is a causative connection there somewhere. I guess if there were sequences which are...
Forum: Sample Prep / Library Generation 12-03-2008, 07:41 PM
Replies: 3
Views: 3,555
Posted By fedora
Discrepancies between PicoGreen and qPCR in GA libraries

Hi all GA users.
Some ChIP libraries we make are giving a ~4x lower concentration by qPCR compared to PicoGreen. These same libraries when sequenced generate low cluster number (~4x less than other...
Forum: Illumina/Solexa 08-26-2008, 06:24 AM
Replies: 5
Views: 5,241
Posted By fedora
Thanks csquared. Have you actually tried this...

Thanks csquared. Have you actually tried this approach? Is there a bioinformatic problem in working out the sequence at the margins of the adapters? Eg how are reads that only have a couple of WGA...
Forum: Illumina/Solexa 08-06-2008, 08:15 PM
Replies: 5
Views: 5,241
Posted By fedora
Sequencing WGA ChIP DNA

I am trying to work out good approach to sequence ChIP DNA that has been amplfied with the GenomePlex Whole Genome Amplification protocol. DNA from this seems to be mostly 400-500bp. Because of the...
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