Forum: Sanger/Dye Terminator
04-16-2019, 08:04 AM
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Replies: 1
Views: 4,726
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Forum: Illumina/Solexa
11-08-2016, 01:27 PM
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Replies: 2
Views: 1,182
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Forum: Illumina/Solexa
08-25-2015, 07:28 AM
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Replies: 2
Views: 1,637
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Forum: Illumina/Solexa
08-21-2015, 09:50 AM
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Replies: 2
Views: 1,637
Odd bioanalyzer results
I had a customer that submitted some antibody libraries which I ran on our MiSeq. The results looked good up until about 100 bases then the Q30 took a nose dive and the %A increased, suggesting that...
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Forum: Illumina/Solexa
02-13-2015, 01:39 PM
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Replies: 2
Views: 1,230
Keeping business from going elsewhere
Don't know if this is the right forum for this question. We have lost a substantial amount of our on campus illumina sequencing business to other service providers (mostly BGI) over the last year. ...
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Forum: Illumina/Solexa
06-27-2014, 09:45 AM
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Replies: 2
Views: 1,437
Constant issues with the HiSeq 2500
We have had a HiSeq 2000/2500 since 2011 that breaks down quite frequently. This round of issues has been going on since around February. It started with no intensity on read 2 of high output runs...
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Forum: Core Facilities
03-03-2014, 01:58 PM
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Replies: 1
Views: 10,573
genotyping by sequencing as service
I have a meeting coming up to discuss the possibility of our core lab offering genotyping by sequencing as a service. I was wondering if anyone who currently offers this service has any advice that...
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Forum: Sample Prep / Library Generation
04-16-2012, 08:53 AM
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Replies: 2
Views: 3,168
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Forum: Sample Prep / Library Generation
04-05-2012, 12:25 PM
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Replies: 2
Views: 3,168
Pippin Prep for TruSeq small RNA libraries
Is anyone using the Pippin Prep to size select their TruSeq small RNA libraries? If so, could you give me information on ease of use, failure rate, and % primer dimer sequences per sample. We are...
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Forum: Sample Prep / Library Generation
03-07-2012, 11:41 AM
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Replies: 1
Views: 3,795
strange library results
I have run 3 sample using TruSeq genomic prep using both gel and non gel based methods. The resulting libraries when run on a bioanalyzer all looked like lane 6 of the attached document. Other...
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