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Forum: 454 Pyrosequencing 11-04-2009, 07:16 AM
Replies: 34
Views: 14,432
Posted By jmbon
Thank you Phillip, this was a quick and...

Thank you Phillip, this was a quick and informative answer. I do not fully follow your point about primers and bleed-over, though. If a primer sequence cause bleed over from well-to-well, many PCR...
Forum: 454 Pyrosequencing 11-02-2009, 09:07 AM
Replies: 34
Views: 14,432
Posted By jmbon
Does a broken T primer help ?

Hello everybody
We had on our first cDNA sequencing try, using a MINT kit used on a 1/4 Titanium plate. No concatenates, but a bimodal length distribution with a first peak around 70 nt and a...
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