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Search: Posts Made By: 454andSolid
Forum: Bioinformatics 05-13-2010, 09:44 AM
Replies: 0
Views: 1,775
Posted By 454andSolid
Merging alignments in mosaik - problems

Hi! I'm having trouble merging alignments in colour space (SOLiD) and base space (454) in Mosaik (version 1.0.1388). This is what I have done:

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## 1. Build Mosaik binary files...
Forum: De novo discovery 05-03-2010, 05:08 AM
Replies: 2
Views: 1,988
Posted By 454andSolid
Thanks for your help! Guess I should get a new...

Thanks for your help! Guess I should get a new manual then. (I have one from October 2008, and I couldn't find the info there...)
Forum: De novo discovery 05-02-2010, 09:57 AM
Replies: 2
Views: 1,988
Posted By 454andSolid
gsAssembler behaving funny with PE data

Hi A couple of questions about genome assembly of 454 using gsAssembler.

Some background: We are sequencing and assembling a eukaryotic genome using 454 titanium data. First we ran 7 slides of...
Forum: Bioinformatics 05-02-2010, 09:40 AM
Replies: 4
Views: 4,410
Posted By 454andSolid
I will try using Nesoni with our transcriptome...

I will try using Nesoni with our transcriptome data.

Thanks for the advice!
Forum: Bioinformatics 04-21-2010, 02:28 AM
Replies: 4
Views: 4,410
Posted By 454andSolid
correcting homopolymer run errors

Hi all,

We have been running de novo assembly of a eukaryotic genome, using 454 titanium together with gsAssembler. When we compare our assembly with cloned cDNA fragments (sequenced with Sanger)...
Forum: Bioinformatics 03-16-2010, 03:22 AM
Replies: 0
Views: 1,372
Posted By 454andSolid
Programs comparing gene sets

Does anybody know of a program to compare different gene sets on the same loci?

This is what I want to do: Our group is doing de-novo sequencing and assembly of a eukaryotic genome. I have run a...
Forum: De novo discovery 01-22-2010, 02:29 AM
Replies: 3
Views: 6,876
Posted By 454andSolid
Thanks! This was exactly what I was looking for....

Thanks! This was exactly what I was looking for. Now I just have to make sure that the contigs with high depth are not mtDNA etc...
Forum: De novo discovery 01-21-2010, 06:48 AM
Replies: 3
Views: 6,876
Posted By 454andSolid
Question Insert size important?

Hi all! First post here, maybe some of you can shed some light on this:

We are working on a denovo sequencing and assembly project, of an eukaryotic genome of ~120 Mbp. So far we have sequenced a...
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