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Search: Posts Made By: rubyryan
Forum: General 05-14-2015, 02:47 PM
Replies: 4
Views: 6,524
Posted By rubyryan
Many thanks Al, thats very helpful and I may try...

Many thanks Al, thats very helpful and I may try this approach, kind regards, Ruby
Forum: General 05-14-2015, 01:20 AM
Replies: 4
Views: 6,524
Posted By rubyryan
Hi Al Merry, I have the same questions and so I...

Hi Al Merry, I have the same questions and so I was wondering if you used the speedvac to concentrate your samples and if it was successful and was also wondering if you were happy using the Turbo...
Forum: Sample Prep / Library Generation 09-24-2012, 08:58 PM
Replies: 18
Views: 12,431
Posted By rubyryan
Many thanks for your comments. Very helpful.

Many thanks for your comments. Very helpful.
Forum: Sample Prep / Library Generation 09-19-2012, 02:30 PM
Replies: 18
Views: 12,431
Posted By rubyryan
Thanks for that! Further to this, once I have...

Thanks for that! Further to this, once I have obtained the ideal total RNA concentration, (200 ng/ul) I am going to try and sequence all of the small RNA (ie microRNA and piwiRNA) using the Illumina...
Forum: Sample Prep / Library Generation 09-18-2012, 01:25 PM
Replies: 18
Views: 12,431
Posted By rubyryan
Many thanks lmolokin for your comments. IF you...

Many thanks lmolokin for your comments. IF you have any advice on concentrating the RNA with minimal loss please let me know as my RNA will need to be concentrated. Thanking you.
Forum: Sample Prep / Library Generation 09-17-2012, 07:09 PM
Replies: 18
Views: 12,431
Posted By rubyryan
RNA extraction and DNase treatment

Hi there,
I am planning to carry out a small RNA sequencing project and would like some advice on the RNA extraction/ DNase treatment protocols. One of my plans is to use the MirVana Paris kit...
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