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Search: Posts Made By: Al Merry
Forum: General 05-14-2015, 01:48 AM
Replies: 4
Views: 6,524
Posted By Al Merry
Hi Ruby, I decided against using the speedvac as...

Hi Ruby, I decided against using the speedvac as I was wary about RNAse contamination. I was very happy using the Turbo DNAse. After tweaking the procedure, I ended up DNAsing using Turbo DNAse, then...
Forum: General 05-17-2013, 09:13 AM
Replies: 4
Views: 6,524
Posted By Al Merry
Talking Speed vac concentration of RNA?

Hi, I have many RNA samples extracted using Ambion Mirvana which I am planning to hybridise to Affymetrix HTA arrays. The protocol requires 150ng RNA in 3ul. My volumes currently range up to about...
Forum: RNA Sequencing 02-27-2013, 09:02 AM
Replies: 31
Views: 28,198
Posted By Al Merry
I have total RNA samples prepared using the...

I have total RNA samples prepared using the Ambion Mirvana kit containing miRNA which I am interested in sequencing, as well as larger RNA species, so I need both small and large RNAs to survive...
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