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Forum: The Pipeline 08-27-2014, 08:50 PM
Replies: 52
Views: 204,502
Posted By gringer
There is now a [pre]-publication out, authored by...

There is now a [pre]-publication out, authored by Nicholas J. Loman and
Aaron R. Quinlan, that introduces a toolkit for working with nanopore data:
...
Forum: The Pipeline 07-14-2014, 09:18 PM
Replies: 52
Views: 204,502
Posted By gringer
A coverage of 30X is not necessary to reduce 1.5M...

A coverage of 30X is not necessary to reduce 1.5M contigs down to something much more manageable -- a single MinION run, even at less than 1X coverage, will be more than a bit useful for cleaning up...
Forum: The Pipeline 07-13-2014, 12:03 AM
Replies: 52
Views: 204,502
Posted By gringer
If money is the limiting factor, rather than...

If money is the limiting factor, rather than time, then carrying out sequencing using a single cartridge is possible, it will just take longer. A single MinION probably won't (yet) produce enough...
Forum: The Pipeline 02-28-2012, 02:32 PM
Replies: 52
Views: 204,502
Posted By gringer
If you read their website, you might notice it's...

If you read their website, you might notice it's actually (2) that they're marketing (I might as well link to it once again). The exonuclease is being done in something approximating a partnership...
Forum: The Pipeline 02-28-2012, 01:33 AM
Replies: 52
Views: 204,502
Posted By gringer
This is not particularly important. Quoting...

This is not particularly important. Quoting myself from this thread:


As long as you have money and monkeys, you can sequence as many bananas as you want. With the "release date" 2000-pore...
Forum: The Pipeline 02-28-2012, 12:49 AM
Replies: 52
Views: 204,502
Posted By gringer
In my post on the "disruptive technology" thread,...

In my post on the "disruptive technology" thread, I came up with about 200kb per pore per hour:

http://seqanswers.com/forums/showpost.php?p=65413&postcount=44

This was from an estimate of...
Forum: The Pipeline 02-25-2012, 01:04 AM
Replies: 52
Views: 204,502
Posted By gringer
Aww, I wanted to hear that the lambda sequencing...

Aww, I wanted to hear that the lambda sequencing was actually done by 5 passes on an Ion Proton.
Forum: The Pipeline 02-19-2012, 01:01 PM
Replies: 52
Views: 204,502
Posted By gringer
I wouldn't say ONT is really going for that...

I wouldn't say ONT is really going for that market yet.[/QUOTE]

The website is surprisingly useful for answering questions about many things related to their method and intentions (hence my...
Forum: The Pipeline 02-19-2012, 06:19 AM
Replies: 52
Views: 204,502
Posted By gringer
I can only guess at the nature of the error that...

I can only guess at the nature of the error that they are talking about, but the "tricky regions" may not necessarily be an obvious class of sequences. Maybe there'll be an issue with only A/T...
Forum: The Pipeline 02-19-2012, 06:04 AM
Replies: 52
Views: 204,502
Posted By gringer
The information on the website suggests that the...

The information on the website suggests that the pores (in solution) are loaded onto the chip at the start of a run:

http://www.nanoporetech.com/technology/single-use-cartridge/sensor-chip


...
Forum: The Pipeline 02-18-2012, 04:29 AM
Replies: 52
Views: 204,502
Posted By gringer
If this is a raw per-molecule error (as I assume...

If this is a raw per-molecule error (as I assume with a "single molecule" technology), then I consider it acceptable. Illumina and SOLiD technologies use hundreds of identical sequencing reactions...
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