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Forum: Illumina/Solexa 08-21-2017, 05:02 AM
Replies: 9
Views: 1,404
Posted By smurugesan
Thank you so much @nucacidhunter. That clears my...

Thank you so much @nucacidhunter. That clears my doubt, thanks again.
Forum: Illumina/Solexa 08-14-2017, 11:06 PM
Replies: 9
Views: 1,404
Posted By smurugesan
Thanks for your explanataion Brian. Its really...

Thanks for your explanataion Brian. Its really helpful. For e.g I have 96 different barcodes in my forward primers, But reverse primer is without barcode, My question is if illumina sequence in both...
Forum: Illumina/Solexa 08-12-2017, 10:06 PM
Replies: 9
Views: 1,404
Posted By smurugesan
Thank you Jessica and Brian for your valuable...

Thank you Jessica and Brian for your valuable suggestions. In Illumina guide, they suggested to sequence an amplicon of 550bp or smaller. So I thought it would be ideal, and actually We are planning...
Forum: Illumina/Solexa 08-09-2017, 04:16 AM
Replies: 9
Views: 1,404
Posted By smurugesan
Great, Thank you. A list of customized forward...

Great, Thank you. A list of customized forward primers with barcodes and Reverse primer without barcode, is it possible to sequence amplicon size of 530bp using 600 v3 kit?
Forum: Illumina/Solexa 08-09-2017, 02:11 AM
Replies: 9
Views: 1,404
Posted By smurugesan
How many number of samples per lane

Hi everyone,

I am a new user to Illumina MI-SEQ, in a single lane how many 16S Amplicons we can mix or in other way, to sequence 1000 samples, how may times we have sequence. Can any one suggest...
Forum: Introductions 08-09-2017, 01:24 AM
Replies: 0
Views: 553
Posted By smurugesan
Hi hello

Hi everyone,

This is selva. I am going to use Illumina Miseq for 16S. Thank you somuch for this forum, it is always helpful to newbies. Keep going.
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