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Search: Posts Made By: Pauline dlp
Forum: General 07-17-2018, 01:51 AM
Replies: 3
Views: 2,019
Posted By Pauline dlp
Thank you for your reply. So if one were to do...

Thank you for your reply. So if one were to do this method, the primer should have the phosphorothioate modification to make it exonuclease resistant, is this right?
Forum: Illumina/Solexa 07-17-2018, 01:46 AM
Replies: 4
Views: 1,571
Posted By Pauline dlp
It seems you are right. The adapters are modified...

It seems you are right. The adapters are modified to have a phosphate group on the 5' and phosphorothioate on the 3' end before the terminal T to protect it from 3' -> 5' exonuclease activity.

I...
Forum: General 07-15-2018, 11:38 PM
Replies: 3
Views: 2,019
Posted By Pauline dlp
Anyone doing GBS/ RAD? A question about using selective primers

So I saw several papers referencing a method used by Sonah (2013) (http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0054603) where they used a selective primer that has one or two...
Forum: Illumina/Solexa 07-15-2018, 11:28 PM
Replies: 4
Views: 1,571
Posted By Pauline dlp
If you already have the sequence, I don't see...

If you already have the sequence, I don't see what additional hurdles you have in ordering the oligos. I'm from the Philippines and we order custom-made oligos from IDT or Macrogen (South Korea)...
Forum: General 04-19-2018, 06:46 AM
Replies: 3
Views: 1,755
Posted By Pauline dlp
Thanks for the reply, nucacidhunter! So it seems...

Thanks for the reply, nucacidhunter! So it seems methylation sensitivity is more of an issue for plants. I tried doing in silico digests of my reference genome with several REs, and the methylation...
Forum: General 04-19-2018, 01:36 AM
Replies: 3
Views: 1,755
Posted By Pauline dlp
Methylation-sensitive restriction enzymes for GBS

Hi all!

I need help in selecting a restriction enzyme for GBS. Elshire's protocol for GBS calls for methylation-sensitive enzymes (They used ApeKI). He also underscores this in GBS workshops. I...
Forum: Introductions 03-19-2018, 03:13 AM
Replies: 0
Views: 1,191
Posted By Pauline dlp
Smile Mabuhay from the Philippines

<first post didn't post for some reason. ho-hum. This is my 2nd time to introduce myself.>

Hello everyone! I'm Pauline, from the Philippines, a graduate of BS Molecular Biology and Biotechnology...
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