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Forum: Illumina/Solexa 01-11-2013, 08:05 AM
Replies: 43
Views: 24,685
Posted By Andrew_Slatter
Note that Fluidigm AccessArray use LNA groups in...

Note that Fluidigm AccessArray use LNA groups in their custom sequencing primers to increase stability without using long oligos.
Forum: De novo discovery 01-11-2013, 08:00 AM
Replies: 6
Views: 2,255
Posted By Andrew_Slatter
Can't you put M13 or NGS primer sequences in a 5'...

Can't you put M13 or NGS primer sequences in a 5' tail to the 3' degeneracy ? That way your amplicons would be directly sequencable with no extra steps
Forum: Illumina/Solexa 01-09-2013, 02:04 AM
Replies: 9
Views: 2,806
Posted By Andrew_Slatter
Thanks for all of your comments. The new training...

Thanks for all of your comments. The new training videos on the illumina website explain it very nicely. Dual index read strategy is particularly interesting.
Forum: Illumina/Solexa 01-02-2013, 08:37 AM
Replies: 9
Views: 2,806
Posted By Andrew_Slatter
So the clusters can be interrogated without...

So the clusters can be interrogated without stripping away either strand ?
Forum: Illumina/Solexa 01-02-2013, 07:27 AM
Replies: 9
Views: 2,806
Posted By Andrew_Slatter
Cluster regeneration

I'm trying to figure out how the MiSeq clustering works with respect to sequencing. Firstly, after the initial cluster generation, both strands are present on the flow cell (i.e. both templates for...
Forum: Ion Torrent 02-16-2012, 03:18 AM
Replies: 2
Views: 7,586
Posted By Andrew_Slatter
We are launching our GenomePooling technology,...

We are launching our GenomePooling technology, that allows users to pool multiplexed tagged genomes of up to 384 samples, followed by region of interest extraction and NGS on any platform. Pooled...
Forum: Illumina/Solexa 10-31-2011, 04:56 AM
Replies: 0
Views: 1,676
Posted By Andrew_Slatter
MiSeq service ?

Anyone have availability on a MiSeq for a 2x150 PE run.
Forum: Illumina/Solexa 06-01-2011, 09:16 AM
Replies: 6
Views: 2,402
Posted By Andrew_Slatter
All depends on what quality you need at the end...

All depends on what quality you need at the end of the run. I've just been looking at the latest MySeq performance data and they report a mean qscore of >20 at 150 bases.
Forum: 454 Pyrosequencing 06-01-2011, 09:03 AM
Replies: 6
Views: 2,149
Posted By Andrew_Slatter
Its hard to say what can be concluded without...

Its hard to say what can be concluded without looking at experimental design.
Have you mapped the reads to your expected target ?
Did you capture the fusion junction by PCR ? If so, you should be...
Forum: 454 Pyrosequencing 06-01-2011, 06:29 AM
Replies: 6
Views: 2,149
Posted By Andrew_Slatter
Yes, read data is 5'-3'. Reads start with TCAG...

Yes, read data is 5'-3'. Reads start with TCAG key which are the last 3' bases of the 454A primer.
Forum: Illumina/Solexa 02-09-2011, 08:10 AM
Replies: 8
Views: 4,259
Posted By Andrew_Slatter
Yes, I am familiar with this paper. They are...

Yes, I am familiar with this paper. They are using their own index sequences within adaptors - and capturing the index sequence as part of a single read. I am interested in the more recent Illumina...
Forum: Illumina/Solexa 02-09-2011, 04:28 AM
Replies: 8
Views: 4,259
Posted By Andrew_Slatter
Alternative index primer

Has anyone used an alternative custom indexing primer on any of the Illumina sequencing platforms ?
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