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Forum: Illumina/Solexa 03-19-2015, 09:44 AM
Replies: 1
Views: 3,079
Posted By acidcoated
Confusion about single end vs paired end read output on Illumina HiSeq

Hello,

I think I'm confused about read output for Illumina's HiSeq. I think I'm not understanding why it is possible to get 150M single end reads in a run, but you can get 300M pairs (is this...
Forum: Sample Prep / Library Generation 07-21-2014, 11:27 AM
Replies: 20
Views: 22,400
Posted By acidcoated
Whoops, saw that you're using the Nextera kit. I...

Whoops, saw that you're using the Nextera kit. I can't read apparently.
Have you tried using the Tn5 transposase through Epicentera without ordering the Nextera kit and does this work for ATAC-seq?
Forum: Sample Prep / Library Generation 07-21-2014, 11:17 AM
Replies: 20
Views: 22,400
Posted By acidcoated
Hi dagarfield, What transposase are you...

Hi dagarfield,

What transposase are you doing the ATAC-seq with? Are you using the Nextera DNA Sample prep kit or ordering the EZ Tn5 through epicentre?
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