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Forum: Sample Prep / Library Generation 01-18-2017, 11:45 PM
Replies: 270
Views: 105,669
Posted By rnaseqmonkey
Did anyone solve the mystery of bacterial rRNA...

Did anyone solve the mystery of bacterial rRNA contaminations yet?
We're also seeing this pop up inversely correlated to the endogenous RNA amount. We use Maxima H- RT for our libraries, too!
Forum: RNA Sequencing 07-28-2016, 01:52 PM
Replies: 2
Views: 1,923
Posted By rnaseqmonkey
RNAprotect Cell has been used for scRNA-seq in...

RNAprotect Cell has been used for scRNA-seq in the SCRB-seq method (Soumillon et al 2014)
It seems to be possible to FACS "fixed" cells, but I hear this is incompatible with antibody stainings.
...
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