Forum: Bioinformatics
04-25-2019, 07:40 AM
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Replies: 0
Views: 1,685
Picard InsertSizeMetrics Output Interpretation
Hi,
I am looking at the data from my pilot Miseq Nano run performed on ATAC-seq libraries.
I wanted to see whether the fragment size distribution pattern corresponds to the one normally observed...
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Forum: Illumina/Solexa
04-24-2019, 07:11 AM
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Replies: 2
Views: 1,560
Thanks for your reply! What exactly are your...
Thanks for your reply! What exactly are your criteria to conclude that the sample "looks good"? Would you say that I don't need to worry if I don't see TSS enrichment in the bigwig file that I've...
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Forum: Illumina/Solexa
04-24-2019, 04:01 AM
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Replies: 2
Views: 1,560
QC of ATAC-seq libraries on MiSeq Nano
Hi all,
I have recently prepared a pool of 9 ATAC-seq libraries from mouse hearts and performed a MiSeq Nano run to validate my pooling calculations, as it's difficult to accurately quantify the...
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Forum: Sample Prep / Library Generation
03-26-2019, 10:15 AM
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Replies: 5
Views: 3,963
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Forum: Illumina/Solexa
03-12-2019, 06:56 AM
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Replies: 1
Views: 2,131
ATAC-seq library sequencing
Hi,
I am about to submit my first mouse ATAC-seq libraries for sequencing, but I have doubts regarding the number of libraries to pool in one 100bp paired-end HiSeq2500 Rapid Run.
The Buenostro...
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Forum: Bioinformatics
02-12-2012, 01:22 AM
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Replies: 2
Views: 1,515
Exclude variants in certain regions
Hi all,
I would be very greatful if somebody can advise me software which could exclude SNPs at known regions. My problem are the SNPs in primer sequences, I have to exclude them out my further...
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Forum: Bioinformatics
02-07-2012, 05:06 AM
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Replies: 4
Views: 5,789
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Forum: Bioinformatics
02-07-2012, 03:20 AM
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Replies: 4
Views: 5,789
VarScan output to VCF
Hi all,
my question might seem simple but I'm new to NGS so please help me..!
I was working with VarScan programme, used "pileup2snp" option. The result is a txt file with a list of SNPs. Now I...
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