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Forum: Illumina/Solexa 10-27-2011, 10:54 AM
Replies: 16
Views: 3,454
Posted By seq_me
I feel the same. at first look ,they look like...

I feel the same. at first look ,they look like PCR duplicates and hence overamplification of the samples. I would contact the person making the libraries and get details on amount of input DNA. If...
Forum: Ion Torrent 10-22-2011, 08:39 PM
Replies: 15
Views: 7,349
Posted By seq_me
Thumbs up Seq analysis time difference

Thanks for the informative article. I was wondering what is difference in processing time for sequence analysis for the 314, 316 and 318 chips.
Forum: Illumina/Solexa 09-08-2011, 01:17 PM
Replies: 7
Views: 2,200
Posted By seq_me
My reply is based on using GaIIx

My reply is based on using GaIIx
Forum: Illumina/Solexa 09-08-2011, 11:08 AM
Replies: 7
Views: 2,200
Posted By seq_me
Aplany, I am trying to answer your question in...

Aplany, I am trying to answer your question in three parts. third part is main explaination.

You can mix pool libraries at different concentration. Theoreticall you will get the reads in the same...
Forum: Sample Prep / Library Generation 07-06-2011, 11:45 PM
Replies: 3
Views: 4,891
Posted By seq_me
Yes t4 polymerase will make the ends blunt and T4...

Yes t4 polymerase will make the ends blunt and T4 PNK will make sure that 5' ends are phosphorylated and 3' ends have the hydroxyl group. I dont think of a reson of not using this esp if your DNA...
Forum: Sample Prep / Library Generation 06-30-2011, 10:59 AM
Replies: 0
Views: 2,184
Posted By seq_me
Library efficiency using Agilent Surelect

I am interested in knowing the efficiency of different library prep methods.

I want to to compare the blunt end ligation with a-tail and especially comparing the different kits available in the...
Forum: Events / Conferences 10-11-2010, 01:54 PM
Replies: 15
Views: 8,974
Posted By seq_me
Presentation Link

In case you missed the meeting or are interested in the presentations of the meeting, please shoot me an email and I will send the username and password
Forum: Events / Conferences 09-07-2010, 03:28 PM
Replies: 15
Views: 8,974
Posted By seq_me
I am interested in going for the evolution of...

I am interested in going for the evolution of next generation sequencing conference. Working in the area, it's time to learn about more applications for next generation sequencing and this seems a...
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