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Forum: Illumina/Solexa 12-11-2018, 02:29 AM
Replies: 4
Views: 2,903
Posted By Brunda
Hello, If you can read this in time - can this be...

Hello, If you can read this in time - can this be done with miseq instrument ? I need to make him sequence dual indexing instead of single indexing, so if you know which files to modify.

Thanks!
Forum: Sample Prep / Library Generation 02-28-2014, 02:13 AM
Replies: 10
Views: 7,337
Posted By Brunda
We're also trying to get more consistent results...

We're also trying to get more consistent results in our lab, so we tried to omit the beads and go for kapa quantification and manual pooling, but what we got was extremely low amount of clusters. I...
Forum: Sample Prep / Library Generation 06-20-2013, 06:04 AM
Replies: 3
Views: 4,155
Posted By Brunda
Hello Carly, how did you resolve your problem? I...

Hello Carly, how did you resolve your problem? I just stumbled onto thread on http://seqanswers.com/forums/showthread.php?t=14511

i wonder if it's possible to do the qubit measurement instead of...
Forum: Sample Prep / Library Generation 06-20-2013, 05:55 AM
Replies: 3
Views: 3,838
Posted By Brunda
No experience with this, but I am curious how...

No experience with this, but I am curious how this works. I've deen doing exomes on SOLiD ald plan to do some more, So maybe this could be a good way to multiplex samples before the exome enrichment...
Forum: SOLiD 03-27-2013, 03:31 AM
Replies: 25
Views: 11,439
Posted By Brunda
Hi, I need your help please. I accidentaly...

Hi, I need your help please.

I accidentaly ordered run cycle buffers and buffer D for solid wildfire, although we do have 5500xl only. Do you think i can use these buffers, or throw them away?...
Forum: SOLiD 02-01-2013, 01:47 AM
Replies: 5
Views: 2,210
Posted By Brunda
You can take a look on control analyzer runs....

You can take a look on control analyzer runs. Sample 1 Is the one with changed temperature. In Sample 2, i tried to replace A-tailing enzyme II with Taq polymerase (which should have the a-tailing...
Forum: SOLiD 01-31-2013, 06:05 AM
Replies: 5
Views: 2,210
Posted By Brunda
I start with 1 ug DNA, End up with about 300 ng...

I start with 1 ug DNA, End up with about 300 ng after size select (concentration 10 ng/ul) after amplification, I got approx. 3 ng/ul and a small peak on analyzer. So it seems that at least some...
Forum: SOLiD 01-31-2013, 05:38 AM
Replies: 5
Views: 2,210
Posted By Brunda
I do control multiNA run (similar to bioanalyzer)...

I do control multiNA run (similar to bioanalyzer) after size selection. Looks pretty much as it should, Yield ~30% of entry amount, 150-180 bp fragment lenght. How would you check if end polishing is...
Forum: SOLiD 01-31-2013, 02:22 AM
Replies: 5
Views: 2,210
Posted By Brunda
Post Automated vs. Manual fragment library preparation - need script!

Hi,
We bought "48 fragment library construction kit" for Solid 5500, which is supposed to be used in automated systems like Tecan, but we don't have one so I am using this kit manually. As I do not...
Forum: Introductions 01-08-2013, 02:55 AM
Replies: 0
Views: 569
Posted By Brunda
hello to all

Hi, I just started working with SOLID 5500 sequencer, I hope this forum will help me to be successful.
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