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Forum: Illumina/Solexa 04-25-2013, 03:04 PM
Replies: 58
Views: 39,560
Posted By Harlon
Hi Kameron Thanks for the link to the...

Hi Kameron

Thanks for the link to the technical bulletin. We had already implemented all of the fixes you suggest, including a maintenance wash between every run, prior to reporting our carry-over...
Forum: Illumina/Solexa 04-22-2013, 08:11 AM
Replies: 58
Views: 39,560
Posted By Harlon
Hi Tom We can say with certainty that we're...

Hi Tom

We can say with certainty that we're getting cross-contamination from previous sequencing runs.

Barcode contamination would give reads on the sequences represented within a run, but...
Forum: Illumina/Solexa 04-17-2013, 09:38 AM
Replies: 58
Views: 39,560
Posted By Harlon
Hi All I just read James' blog post on this:...

Hi All

I just read James' blog post on this:
http://core-genomics.blogspot.ca/2013/04/miseq-and-2500-owners-better-read-this.html
I'm not certain that my original statement of the problem is...
Forum: Illumina/Solexa 04-10-2013, 08:23 AM
Replies: 58
Views: 39,560
Posted By Harlon
We've always run 0.5% Tween in our maintenance...

We've always run 0.5% Tween in our maintenance washes, and we do switch buffers in both the bottle and the tray in between each step of the wash. We also almost always do our post-run wash within a...
Forum: Illumina/Solexa 04-08-2013, 05:02 PM
Replies: 58
Views: 39,560
Posted By Harlon
MiSeq Carry-over contamination between runs

Hi All

We appear to be suffering from carry over contamination in our MiSeq runs - i.e. if we sequence a DNA sample in one MiSeq run, we see about the same sample in the subsequent run.
...
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