Forum: Bioinformatics
11-26-2013, 08:03 AM
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Replies: 7
Views: 3,777
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Forum: Bioinformatics
11-26-2013, 07:03 AM
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Replies: 7
Views: 3,777
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Forum: 454 Pyrosequencing
07-05-2013, 12:30 AM
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Replies: 2
Views: 2,284
Amplicon sequencing - fusion primers ordering
Dear all,
we would like to order fusion primers for 454 GS Junior amplicon sequencing.
When consulting the manual, we are not sure if the primers need to have Phosphorothioated DNA bases on the...
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Forum: Illumina/Solexa
02-11-2013, 04:24 PM
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Replies: 1
Views: 1,767
Hi Miu,
I have just experience the same...
Hi Miu,
I have just experience the same thing, fragmenting pure viral RNA. I`ve tried 6,7,8,9,10 min with this buffer and also different times (10, 15 min) with RNAse III module. Also, I always...
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Forum: Illumina/Solexa
02-11-2013, 02:26 PM
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Replies: 3
Views: 2,078
Thank you,
kcchan: you`ve done it for the...
Thank you,
kcchan: you`ve done it for the directional RNA sequencing protocol?
Anyway I have to cut the library band from the gel in the last step, my fear is only that I would not be able to see...
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Forum: Illumina/Solexa
02-10-2013, 11:34 AM
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Replies: 3
Views: 2,078
Low input directional viral RNA sequencing
Dear users,
I would like to use directional RNA sequencing protocol (with TrueSeq siRNA adapters) to prepare libraries from fragmented RNA of purified virus. After the fragmentation and end repair...
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Forum: Illumina/Solexa
01-09-2013, 08:20 AM
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Replies: 2
Views: 1,137
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Forum: Illumina/Solexa
01-09-2013, 04:24 AM
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Replies: 2
Views: 1,137
Multiplexing libraries of slightly different size
Hi everybody,
I would like to multiplex libraries of different size, in one case siRNA libraries (150 bp) and in other slightly bigger ones (around 200 bp).
Is the difference in size acceptable...
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Forum: Bioinformatics
12-07-2012, 08:31 AM
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Replies: 0
Views: 901
MAQ pileup problem
Dear all,
I'm processing MAQ pileup file and I have a problem: the depth of coverage value, which MAQ gives me is sometimes different from the one I get just counting the characters covering the...
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Forum: Bioinformatics
12-04-2012, 11:44 AM
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Replies: 2
Views: 813
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Forum: Bioinformatics
12-04-2012, 09:31 AM
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Replies: 2
Views: 813
position heterogenity from SAMtools pileup?
Dear users,
I would like to get report, which would give me the information what's the portion of each nucleotide, which have been mapped to each position in a ref sequence, something like:
1....
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