Forum: Bioinformatics
05-14-2012, 09:38 AM
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Replies: 0
Views: 1,723
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Forum: Bioinformatics
12-28-2010, 10:10 PM
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Replies: 1
Views: 3,310
How to map SOLiD paired end reads by Bfast
1. I found that bfast localalign can't support -1 and -2 options. So I'm confused how it works in
http://seqanswers.com/forums/showthread.php?t=7100
My bfast version is 0.6.4e. Is -1 and -2 work...
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Forum: Bioinformatics
12-07-2009, 03:04 AM
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Replies: 3
Views: 2,086
reads mapping to overlapping genes?
:confused:
Many reads are mapped to positions where overlapping genes reside. So we overestimated the number of reads mapping to some genes. How to deal with this problem?
Thank you for your...
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Forum: Bioinformatics
12-01-2009, 01:37 AM
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Replies: 23
Views: 25,082
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Forum: Bioinformatics
11-30-2009, 06:48 PM
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Replies: 23
Views: 25,082
how to study differential expression?
My data is got from 2 tissues by Illumina sequencing with 75nt reads. Are there any standard ways to study differential expression?
Is it necessary to calculate RPKM for each gene? If so what is...
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Forum: Illumina/Solexa
11-27-2009, 06:43 PM
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Replies: 2
Views: 1,973
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Forum: Illumina/Solexa
11-26-2009, 07:12 AM
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Replies: 2
Views: 1,973
the meaning of Match Position for reads?
hi ^_^
In the s_N_export.txt file, If the Match Position for one read is x, should the other end position of this read be x+75 both for "F" forward and "R" reverse strand match? (75 is the read...
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