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Search: Posts Made By: Claudia Stewart
Forum: Ion Torrent 05-31-2011, 08:07 AM
Replies: 18
Views: 6,053
Posted By Claudia Stewart
Apparently, the issue is the micelle size –...

Apparently, the issue is the micelle size – material larger than 250bp doesn’t drive back to bead well. I suspect that this will no longer be the case with the new One Touch, since there is no oil...
Forum: Ion Torrent 05-31-2011, 07:54 AM
Replies: 10
Views: 3,396
Posted By Claudia Stewart
We have had our PGM since the end of Frebruary,...

We have had our PGM since the end of Frebruary, made 48 runs and still have not needed to replace. We were told that we could expect to replace the tank every 4-6 weeks if we ran it every day.
Forum: 454 Pyrosequencing 10-09-2009, 07:07 AM
Replies: 21
Views: 8,910
Posted By Claudia Stewart
emPCR problems

Yes, we have - we were told they are 'unseen broken reactors', meaning that even though these were inspected at emPCR completion and found to be non- biphasic (ie not obviously broken) that there can...
Forum: 454 Pyrosequencing 08-30-2009, 10:47 AM
Replies: 7
Views: 1,691
Posted By Claudia Stewart
Has anyone seen a very high percentage of dots...

Has anyone seen a very high percentage of dots whenlooking at tandem repeats (80%)? Can you adjust the reaction to read through these and/or capture the data back from the filter to analyse what is...
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