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Forum: Sample Prep / Library Generation 07-16-2015, 09:13 PM
Replies: 4
Views: 1,298
Posted By PhatB
Maybe the pooling method would be too much...

Maybe the pooling method would be too much handling and lead to potential degradation? The core facility at my research institute uses Clonetech's ultralow kit that contains a 10x reaction buffer for...
Forum: Sample Prep / Library Generation 07-15-2015, 02:06 PM
Replies: 4
Views: 1,298
Posted By PhatB
Pooling FACS sorted single cells to increase yield - Best method to concentrate RNA?

Hey all,

I am attempting to pool RNA from single cells that have been FACS sorted in an attempt to increase my RNA concentration before cDNA amplification and library prep. The reason I have to...
Forum: Sample Prep / Library Generation 05-29-2015, 12:30 PM
Replies: 2
Views: 3,153
Posted By PhatB
RNA from FACS-sorted Para-fixed cells for RNA-seq

Hey all,

I have recently attempted to FACS sort virally infected mouse splenocytes that were fixed and stained with intracellular flow antibodies. I yielded about 1.5 million cells of each...
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