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Search: Posts Made By: lorenzo.C
Forum: Bioinformatics 02-13-2020, 01:16 PM
Replies: 3
Views: 927
Posted By lorenzo.C
isnt this command comparing a bam file to the...

isnt this command comparing a bam file to the reference genome? what I want are the SNV/indels present in the mutant strain but not in the control strain.
Forum: Bioinformatics 02-13-2020, 10:28 AM
Replies: 3
Views: 927
Posted By lorenzo.C
find variants compared to sequenced reference

hi folks, I have 5 illumina sequencing fastq files. 1 is the parent and 4 are mutants. I alligned the sequence to the refenrence genome I have all the BAM bed etc etc files. I cleaned repetitive seq...
Forum: Bioinformatics 06-27-2019, 11:22 AM
Replies: 4
Views: 1,036
Posted By lorenzo.C
thanks for the help good sir, if I understand...

thanks for the help good sir, if I understand correctly --al-conc <path> will produce a new couple of fastq that fulfil the --maxins option. the maxins command will take just reads that are spaced to...
Forum: Bioinformatics 06-26-2019, 09:56 AM
Replies: 4
Views: 1,036
Posted By lorenzo.C
BOWTIE2 would be great! thanks for the help!

BOWTIE2 would be great! thanks for the help!
Forum: Bioinformatics 06-21-2019, 11:39 AM
Replies: 4
Views: 1,036
Posted By lorenzo.C
isolate reads from a paired-ended fastq files

hi folks,
I have pair ended fastq files (let's call them 01_R1 and 01_R2). I need to make a new pair of fastq files that contains just the reads that fulfill this requirement: the reads of R2 should...
Forum: General 01-22-2018, 11:02 AM
Replies: 0
Views: 1,257
Posted By lorenzo.C
NSG to detect deletions and duplications genome-wide

hi there,

I need to detect deletions and duplications genome-wide in human cells. ideally I would like to use several clones (the more the better) and detect the number of deletions and...
Showing results 1 to 6 of 6

 


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