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Forum: 454 Pyrosequencing 08-12-2009, 02:19 PM
Replies: 26
Views: 44,830
Posted By mccallumj
re Poly A etc

We have had very nice results from Ti sequencing of normalised cDNA prepared using Evrogen Mint + Trimmer kits, using a modified 3' Primer XXXXXXXXXXX(T)4G(T)9C(T)10VN as described by Beldade et al....
Forum: 454 Pyrosequencing 06-08-2009, 02:23 PM
Replies: 26
Views: 44,830
Posted By mccallumj
PTS Wisdom?

Hi
We are currently working on de novo RNA-seq and re-sequencing in a pre-genome species (onion) and are interested in PTS or a similar generic barcoding approach to enable us to do population...
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