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Forum: Oxford Nanopore 08-11-2017, 09:41 AM
Replies: 7
Views: 4,498
Posted By JonB
That's also the case for me. I work on an algae...

That's also the case for me. I work on an algae and I struggle to get a lot of DNA due to sub-optimal cultures. But the DNA I have is of really high quality though.

Thanks for all the suggestions....
Forum: Oxford Nanopore 08-10-2017, 11:32 PM
Replies: 7
Views: 4,498
Posted By JonB
Thanks! Yes, I was not sure about which order...

Thanks!
Yes, I was not sure about which order to do things (assembly and correction). But your suggestion is very helpful.
Forum: Oxford Nanopore 08-10-2017, 10:48 PM
Replies: 7
Views: 4,498
Posted By JonB
Thanks for the manuscript! I'm not exactly sure...

Thanks for the manuscript!
I'm not exactly sure about the Illumina coverage at the moment, but it's very high at least. Mostly I was concerned about using only 1D because of the error rate, but I...
Forum: Oxford Nanopore 08-08-2017, 11:38 PM
Replies: 7
Views: 4,498
Posted By JonB
Assembly using Illumina + Nanopore 1D reads?

Hi,

I am trying to assemble a eukaryotic genome of about 300MB. I have Illumina data, and I am thinking of trying out the MinION Basic Starter Pack to use for scaffolding. But it produces only 1D...
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