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Forum: Illumina/Solexa 10-15-2021, 02:09 AM
Replies: 0
Views: 481
Posted By XmaX
Miseq LaneQC and Flow Cell failed - but Data By Cycle looks correct

Just finished my first Miseq run, and it appears it failed. Both Lane QC and Flow Cell are listed as failed. The total data is <1GB, even though it was significantly bigger while running.

What...
Forum: Illumina/Solexa 09-22-2021, 07:51 AM
Replies: 1
Views: 758
Posted By XmaX
What indexing should I use?

I am planning a MiSeq experiment in which I will be doing a PCR mutagenesis of a sequence, with the intention of obtaining just a single mutation in the ~100bp sequence. To do this, I will test 9...
Forum: Illumina/Solexa 09-20-2021, 07:02 AM
Replies: 1
Views: 795
Posted By XmaX
Do I need to use a library preparation kit with MiSeq?

Hello everyone.

I am very new to NGS (and to libraries in general) and so I have a few basic questions.

I am planning an experiment in which I will have a number PCR reactions with the...
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