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Forum: Vendor Forum 07-06-2017, 07:12 AM
Replies: 0
Views: 696
Posted By Bioo Scientific
Improved small RNA-Seq from plant total RNA

Learn how to increasing ligation efficiency and discovery of miRNAs with plant samples by reading our recent blog post...
Forum: Vendor Forum 06-29-2017, 11:02 AM
Replies: 0
Views: 477
Posted By Bioo Scientific
Do UMIs reduce small RNA-Seq bias?

Our recent blog post (http://blog.bioongs.com/do-umis-reduce-small-rna-sequencing-bias/) explores strategies for reducing bias in small RNA sequencing, including the use of UMIs and randomized...
Forum: Sample Prep / Library Generation 08-23-2016, 02:25 PM
Replies: 4
Views: 720
Posted By Bioo Scientific
Hi Yue, We would be happy to help you with...

Hi Yue,

We would be happy to help you with this. Can you contact Bioo Scientific’s technical service at [email protected] or 512-707-8993?

Regards,
Bioo Scientific
Forum: Vendor Forum 06-24-2016, 07:32 AM
Replies: 0
Views: 1,129
Posted By Bioo Scientific
BRCA1/2 Amplicon Panel for Detection of Mutations in FFPE Samples

The NEXTflex™ BRCA1 and BRCA2 Amplicon Panel for FFPE Illumina-Compatible (http://www.biooscientific.com/NEXTflex-BRCA1-and-BRCA2-Amplicon-Panel-for-FFPE-Illumina-compatible) can be used to detect...
Forum: Vendor Forum 05-27-2016, 12:40 PM
Replies: 0
Views: 1,365
Posted By Bioo Scientific
Validated, Custom Amplicon Panels for Illumina and Ion Torrent Sequencing

The NEXTflex™ Custom Amplicon Panels (http://www.biooscientific.com/NEXTflex-Custom-Amplicon-Panels)are fully customizable amplicon-based assays for targeted resequencing on the Illumina or Ion...
Forum: Vendor Forum 05-27-2016, 07:53 AM
Replies: 10
Views: 6,205
Posted By Bioo Scientific
Hi Dan, Small RNA sequencing is a little...

Hi Dan,

Small RNA sequencing is a little different in that it involves the ligation of single stranded adapters directly to RNA molecules, rather than conversion to dsDNA and ligation of Y-shaped...
Forum: Vendor Forum 05-18-2016, 09:54 AM
Replies: 0
Views: 1,179
Posted By Bioo Scientific
Cell Free DNA Isolation from Urine

The NextPrep-Mag™ Urine cfDNA Isolation Kit (http://www.biooscientific.com/NextPrep-Mag-Urine-cfDNA-Isolation-Kit) is an automation-friendly kit designed to extract DNA from urine, using a magnetic...
Forum: Vendor Forum 05-17-2016, 09:40 AM
Replies: 0
Views: 805
Posted By Bioo Scientific
Using the NEXTflex Small RNA-Seq Kit v3 for RIP-Seq, CLIP-Seq and Ribosomal Profiling

A number of our customers have asked for a protocol which would enable them to use the NEXTflex™ Small RNA Seq Kit v3 for ribosomal profiling, RIP-Seq (RNA binding protein immunoprecipitation and...
Forum: Vendor Forum 02-12-2016, 12:43 PM
Replies: 0
Views: 871
Posted By Bioo Scientific
Pooling Recommendations for SureSelect Target Capture

Bioo Scientific’s latest Tech Tip, Pooling Recommendations for SureSelect Target Capture...
Forum: Vendor Forum 12-10-2015, 08:20 AM
Replies: 6
Views: 2,521
Posted By Bioo Scientific
Hi Mama, It is unusual to see such a high...

Hi Mama,

It is unusual to see such a high percentage of adapter-dimer product in your libraries. We would be happy to help you troubleshoot this. Can you send the following information to our tech...
Forum: Vendor Forum 12-02-2015, 03:11 PM
Replies: 6
Views: 2,521
Posted By Bioo Scientific
Hi Nucacidhunter, Thanks for the question....

Hi Nucacidhunter,

Thanks for the question. The work by NEB is certainly interesting. It looks like the only original findings are that bias can also be reduced by using either terminal or internal...
Forum: Vendor Forum 10-20-2015, 07:46 AM
Replies: 0
Views: 870
Posted By Bioo Scientific
Low-input, High Multiplexing Library Prep Solution for SeqCap Target Capture

The NEXTflex™ Rapid Pre-Capture Combo Kit (NimbleGen SeqCap Compatible) (http://www.biooscientific.com/NEXTflex-Rapid-Pre-Capture-Combo-Kit-for-SeqCap-Target-Capture) is a complete low-input library...
Forum: Vendor Forum 09-01-2015, 12:55 PM
Replies: 0
Views: 907
Posted By Bioo Scientific
Automated Sciclone protocol for small RNA-Seq library prep is available

A protocol is now available which automates the NEXTflex™ Small RNA-Seq Kit V2 on the Sciclone Workstation, to simplify small RNA-Seq while offering the most accurate small RNA-Seq profiling...
Forum: Vendor Forum 09-01-2015, 06:49 AM
Replies: 2
Views: 1,671
Posted By Bioo Scientific
Hi Anne, Yields and purity are similar. We...

Hi Anne,

Yields and purity are similar. We have data posted on our website comparing the NEXTprep-Mag cfDNA Isolation Kit to the QIAamp Circulating Nucleic Acid Kit...
Forum: Illumina/Solexa 08-03-2015, 10:33 AM
Replies: 10
Views: 2,046
Posted By Bioo Scientific
Bioo Scientific has launched 384 single-index...

Bioo Scientific has launched 384 single-index barcoded adapters (http://www.biooscientific.com/NEXTflex-HT-Barcodes-for-Illumina-Multiplexing) which are validated by sequencing. These barcodes...
Forum: Vendor Forum 07-21-2015, 10:28 AM
Replies: 0
Views: 1,197
Posted By Bioo Scientific
384 single-index, Illumina-compatible adapters

Bioo Scientific recently launched the NEXTflex-HT™ Barcodes, single-index barcodes (http://www.biooscientific.com/NEXTflex-HT-Barcodes-for-Illumina-Multiplexing) added during the ligation step of...
Forum: Sample Prep / Library Generation 07-21-2015, 09:56 AM
Replies: 5
Views: 2,654
Posted By Bioo Scientific
Isolating 5 mL at a time is achievable. For 10 mL...

Isolating 5 mL at a time is achievable. For 10 mL you'll need to perform two cfDNA isolations from plasma.
Forum: Sample Prep / Library Generation 07-20-2015, 01:40 PM
Replies: 5
Views: 2,654
Posted By Bioo Scientific
Bioo Scientific's NextPrep-Mag™ cfDNA Isolation...

Bioo Scientific's NextPrep-Mag™ cfDNA Isolation Kit (http://www.biooscientific.com/NEXTprep-Mag-cfDNA-Isolation-Kit) should be able to help you. It is designed for extracting cfDNA from human blood...
Forum: Vendor Forum 06-16-2015, 07:46 AM
Replies: 1
Views: 1,334
Posted By Bioo Scientific
Now that Illumina Experiment Manager (IEM) v1.9...

Now that Illumina Experiment Manager (IEM) v1.9 supports sample sheet generation for sequencing runs with more than 96 samples in a single sheet, Bioo Scientific has also created instructions...
Forum: Vendor Forum 06-04-2015, 12:59 PM
Replies: 2
Views: 1,671
Posted By Bioo Scientific
Fast, Scalable, Automatable Cell Free DNA Isolation Kit for NGS Library Prep

The NextPrep-Mag™ cfDNA Isolation Kit (http://www.biooscientific.com/NEXTprep-Mag-cfDNA-Isolation-Kit)is designed for extracting cell-free DNA (cfDNA) from human blood plasma or serum for NGS...
Forum: Vendor Forum 05-20-2015, 11:36 AM
Replies: 0
Views: 783
Posted By Bioo Scientific
mtDNA isolation and library prep

The NEXTflex™ mtDNA-Seq Kit (http://www.biooscientific.com/Mitochondrial-DNA-Library-Prep-Kit-for-Illumina/NEXTflex-mtDNA-Seq-Kit) includes optimized reagents for the isolation of mitochondrial...
Forum: Sample Prep / Library Generation 05-19-2015, 02:48 PM
Replies: 11
Views: 2,545
Posted By Bioo Scientific
Hi Ana, You can see the desired product...

Hi Ana,

You can see the desired product size of a NEXTflex 16S V4 Amplicon-Seq library on page 11 of the protocol...
Forum: Illumina/Solexa 03-31-2015, 08:02 AM
Replies: 7
Views: 6,780
Posted By Bioo Scientific
Hi Quibianka, We use T4 RNA Ligase (not...

Hi Quibianka,

We use T4 RNA Ligase (not truncated) to ligate the 5’adapter. /
Forum: Vendor Forum 02-25-2015, 11:25 AM
Replies: 0
Views: 1,172
Posted By Bioo Scientific
Optimized Cell Free Library Prep for Ion Torrent-Based Platforms

Bioo Scientific has just launched the NEXTflex™ Cell Free DNA Sequencing Kit for Ion PGM™ and Ion Proton™ (http://www.biooscientific.com/NEXTflex-Cell-Free-DNA-Seq-Kit-for-Ion-PGM) today. It is...
Forum: Vendor Forum 02-10-2015, 09:01 AM
Replies: 1
Views: 1,488
Posted By Bioo Scientific
Read the whitepaper, Improved Library Prep Offers...

Read the whitepaper, Improved Library Prep Offers Higher Percentage of On-target Reads and Better Coverage for SureSelectXT2 Target Capture...
Showing results 1 to 25 of 99

 


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