Forum: General
06-23-2021, 02:54 AM
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Replies: 0
Views: 2,919
Seq Instrumentation/ Run costs
Hi,
This is a 'piece of string' question in trying to identify the 'ideal' instrument. Is there a well curated review/ discussion anywhere of current instrumentation capital costs (list prices,...
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Forum: Illumina/Solexa
02-27-2020, 02:15 AM
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Replies: 4
Views: 3,412
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Forum: Illumina/Solexa
02-20-2020, 02:40 AM
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Replies: 4
Views: 3,412
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Forum: Ion Torrent
04-10-2018, 07:53 AM
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Replies: 1
Views: 3,687
Droplets, beads & wells
Hi,
Can anyone point me in the direction of a post or publication that lists how the droplet size for emPCR, the bead size and the well dimensions of the various Ion chips vary? Are all of the...
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Forum: Illumina/Solexa
10-03-2017, 06:30 AM
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Replies: 4
Views: 2,097
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Forum: Illumina/Solexa
10-03-2017, 05:48 AM
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Replies: 4
Views: 2,097
Perhaps I could come at this from a different...
Perhaps I could come at this from a different direction (or invite educated guesses?). I am not actually an Illumina user, so I'm not familiar with the workflow that generates the clusters in the...
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Forum: Sample Prep / Library Generation
10-02-2017, 01:14 AM
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Replies: 1
Views: 1,499
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Forum: SOLiD
10-02-2017, 01:00 AM
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Replies: 4
Views: 19,031
I realise that this is replying to a very old...
I realise that this is replying to a very old Thread, but my understanding is that Wildfire was the clonal amplification technology applied to SOLiD, whereas Avalanche was the (same) technology...
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Forum: Illumina/Solexa
10-02-2017, 12:49 AM
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Replies: 4
Views: 2,097
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Forum: Sample Prep / Library Generation
10-02-2017, 12:39 AM
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Replies: 1
Views: 1,499
Clonal Amplification/ Kinetic Exclusion
Newer generation Illumina platforms with patterened flowcells use kinetic exclusion to achieve a high degree of clonal chastity. From what I understand, the amplification method is still bridge, but...
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Forum: Bioinformatics
09-20-2016, 01:39 AM
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Replies: 3
Views: 3,364
Physical removal of adapter/ primer sequences
Slightly off topic, but definitely related, I've been doing a little hunting on the physical removal of the remnants of adapters or, more likely, the primer remnants of a library generated by...
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