Forum: Illumina/Solexa
10-16-2019, 07:20 AM
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Replies: 2
Views: 2,761
NextSeq underclustering
Has anyone had issues with underclustering on the Illumina NextSeq 500/550? We have been having issues all summer. Even increasing input 3 times our normal has not caused overclustering. We...
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Forum: Illumina/Solexa
05-05-2017, 05:39 AM
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Replies: 6
Views: 3,527
Sequal prep ...
Sequal prep
https://www.thermofisher.com/order/catalog/product/A1051001
This is how we normalize.
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Forum: Sample Prep / Library Generation
11-17-2015, 06:56 AM
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Replies: 0
Views: 2,248
Alternatives to SequalPrep
Hi,
I am looking for alternatives to SequalPrep as they are on backorder for a few months and we are in busy mode.
Thanks
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Forum: Illumina/Solexa
05-27-2015, 09:41 AM
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Replies: 2
Views: 1,608
I use the Kozich et al. primer set. I have...
I use the Kozich et al. primer set. I have never seen anything like this.
Could you give more info on the library generation?
Lately the reagents for the MiSeq has been crap and my Q30 have...
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Forum: Sample Prep / Library Generation
02-24-2015, 08:24 AM
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Replies: 19
Views: 7,543
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Forum: Sample Prep / Library Generation
02-22-2015, 07:59 AM
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Replies: 19
Views: 7,543
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Forum: Sample Prep / Library Generation
02-22-2015, 07:58 AM
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Replies: 19
Views: 7,543
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Forum: Sample Prep / Library Generation
12-18-2014, 01:39 PM
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Replies: 3
Views: 5,185
EpMotion - NEBnext ultra program
Was wondering if there is a program out there for genomic library prep on the EpMotion. I know they have the TruSeq RNA one's, but I am more looking for DNA and hoping specfically for NEBNext Ultra....
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Forum: Sample Prep / Library Generation
12-10-2014, 09:27 AM
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Replies: 19
Views: 7,543
For subnanomolar libraries we use the protocol...
For subnanomolar libraries we use the protocol found in the supplemental section (Protocol 12) in the paper below. Its a fantastic protocol and I have used it for libraries with 100 pM (diluted to 4...
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Forum: Core Facilities
12-10-2014, 07:09 AM
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Replies: 22
Views: 25,465
I like the epMotion 5075. I think its a great...
I like the epMotion 5075. I think its a great little robot, especially if you are new to programming these types of devices. I like that it checks to make sure the proper labware is being used and...
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