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Forum: Illumina/Solexa 11-21-2011, 06:50 AM
Replies: 3
Views: 5,856
Posted By poorphd
no, not that simple

In classical biology, we define "Forward" is the direction from 5' to 3' and "Reverse". 3' to 5'. but in NGS sequencing, all the sequences direction is from 5' to 3' , so F means sense strand, and R...
Forum: Bioinformatics 11-04-2011, 01:19 PM
Replies: 6
Views: 4,697
Posted By poorphd
sam flag is confusing

i mapped reads to my transcrpitome assemblied from those reads.
there is a record in the sam file
HWI-ST397_0000:5:1101:19602:2158#ATCTCG 20 UN57620 131 25 95M * ...
Forum: Illumina/Solexa 10-18-2011, 07:50 AM
Replies: 3
Views: 5,856
Posted By poorphd
how to define a forward or reverse read file

i often meet the concept of "forward" or "reverse".
but not exact definition provided

does the "forward" means the reads who have the same direction with PCR primer 1 ?
does the "reverse". means...
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