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Forum: Illumina/Solexa 11-12-2010, 10:30 AM
Replies: 5
Views: 3,675
Posted By acpatel
kit availability? cost?


Any word on how the kit performs? Cost? Availability/

Forum: Introductions 11-03-2010, 09:27 AM
Replies: 6
Views: 3,589
Posted By acpatel
specific question


Could Prelude be used with OCT-embedded tissue samples (very tiny tissue samples)? For OCT-embedded frozen samples, would one use:
OvationŽ RNA-Seq FFPE System
OvationŽ RNA-Seq ...
Forum: Bioinformatics 11-03-2009, 11:14 AM
Replies: 42
Views: 12,318
Posted By acpatel
how much data?


How much data is a "chromosome's worth"? (I ask only because some chromosomes are huge, and others not so huge).

I've got 9 reads, 312-385 average read length, 110-170 mbases per read.
Forum: Bioinformatics 11-03-2009, 10:54 AM
Replies: 42
Views: 12,318
Posted By acpatel
454 support?

Trimming all of the sequences to the same length seems like a bad idea. Any idea when tophat / bowtie will support 454 variable length reads?

Greg -- how did you take your mouse reads and get...
Forum: RNA Sequencing 06-29-2009, 10:26 AM
Replies: 0
Views: 2,555
Posted By acpatel
processing 454 rna-seq data

OK. I have rna-seq data from 3 mice. I want to align this data to the mouse reference -- either genome, which seems like overkill, or to all the known mRNAs from Acembly -- which I have in FASTA...
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