SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 10 of 10
Search took 0.00 seconds.
Search: Posts Made By: phyllosphere
Forum: Illumina/Solexa 01-07-2016, 02:44 PM
Replies: 13
Views: 3,266
Posted By phyllosphere
Thanks @GenoMax. Yes I read almost all the...

Thanks @GenoMax. Yes I read almost all the threads related to MiSeq here (desperate to move on with our microbiome projects). We did get four separate good 2x300 runs with low diversity libraries on...
Forum: Illumina/Solexa 01-07-2016, 01:46 PM
Replies: 13
Views: 3,266
Posted By phyllosphere
On another poor run at end of 2015: cluster...

On another poor run at end of 2015: cluster density (775 +/- 49 k/mm2) with 81.8% PF. Summary graphs attached.
Forum: Illumina/Solexa 01-07-2016, 01:10 PM
Replies: 13
Views: 3,266
Posted By phyllosphere
No problem with previous runs in 2013 and 2014,...

No problem with previous runs in 2013 and 2014, but could not get high quality sequences since 2015.
Forum: Illumina/Solexa 01-07-2016, 12:03 PM
Replies: 13
Views: 3,266
Posted By phyllosphere
The library is the mixtures of 16S and ITS...

The library is the mixtures of 16S and ITS amplicons. We prepared the library following The Schloss Lab Protocol and we had no problem for previous runs -even at 5% PhiX.
Forum: Illumina/Solexa 01-07-2016, 11:57 AM
Replies: 13
Views: 3,266
Posted By phyllosphere
PhiX was added at 30%. Is there a way to tell...

PhiX was added at 30%. Is there a way to tell whether the PhiX added was bad (degraded)?
Forum: Illumina/Solexa 01-07-2016, 07:25 AM
Replies: 13
Views: 3,266
Posted By phyllosphere
Here is the message from Illumina: "I can't...

Here is the message from Illumina:
"I can't say for sure if the run is over-clustered, but it does look like it is low diversity and perhaps a lack of PHiX caused it to loose focus. Depending on...
Forum: Illumina/Solexa 01-07-2016, 07:20 AM
Replies: 13
Views: 3,266
Posted By phyllosphere
No clusters or low intensity clusters on MiSeq runs

We are experiencing problems with MiSeq runs (using 600-cycle V3 kits) at our core facility: Two week ago they ran a PhiX standard and a customer-prepared library- both worked fine. However, when...
Forum: Illumina/Solexa 12-23-2015, 12:29 PM
Replies: 4
Views: 2,040
Posted By phyllosphere
Good to know-thanks!

Good to know-thanks!
Forum: Illumina/Solexa 12-23-2015, 06:43 AM
Replies: 4
Views: 2,040
Posted By phyllosphere
Thank you! Will it be possible to mix our MiSeq...

Thank you! Will it be possible to mix our MiSeq libraries with our HiSeq libraries (e.g., RNA-Seq, have not started yet) and running the mix libraries with HiSeq2500 or 3000?
Forum: Illumina/Solexa 12-22-2015, 01:56 PM
Replies: 4
Views: 2,040
Posted By phyllosphere
MiSeq v3 kit for high diversity vs. low diversity libraries

Like many folks working on low diversity libraries, we have been hit hard by the problems associated with the V3 kit - lots of time and effort wasted. Is this problem also common for high diversity...
Showing results 1 to 10 of 10

 


All times are GMT -8. The time now is 02:40 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO