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Forum: Metagenomics 11-04-2014, 06:34 AM
Replies: 13
Views: 4,733
Posted By Richa Sharma
I understand, for PCA, I must provide a metadta...

I understand, for PCA, I must provide a metadta file. But I wanted to start with plotting simple bar graphs. where is the problem. Can you tell me ?
Forum: Metagenomics 11-04-2014, 05:05 AM
Replies: 13
Views: 4,733
Posted By Richa Sharma
No I did not include a metadeta file.

No I did not include a metadeta file.
Forum: Metagenomics 11-04-2014, 04:06 AM
Replies: 13
Views: 4,733
Posted By Richa Sharma
Hi overflow, Thanks for help. I work with...

Hi overflow,

Thanks for help. I work with windows. I installed STAMP, and I was also able to make SPF file (from mothur files) using it, but when I try to load them, I cant see anything on STAMP...
Forum: Metagenomics 11-03-2014, 05:59 AM
Replies: 13
Views: 4,733
Posted By Richa Sharma
Hi overflow, Thanks for your suggestion. I...

Hi overflow,

Thanks for your suggestion. I dint hear about STAMP before. Are you sure if I can use it for mothur generated data? Because I did not see any explanation of mothur data file format in...
Forum: Metagenomics 10-29-2014, 02:49 AM
Replies: 13
Views: 4,733
Posted By Richa Sharma
Question How to visualize microbiome datasets ?

Hi,

I have 16S rRNA datasets produced by Mothur and I want to plot my data and get publictaion quality figures (stacked bars, heatmaps, etc). I dont have familiarity with command line based...
Forum: Illumina/Solexa 02-19-2014, 01:10 AM
Replies: 23
Views: 23,602
Posted By Richa Sharma
Illumina Miseq V4-V5 16S rRNAsequencing

Hi all,

This is Richa. I have sent my 16s V4-V5 amplicons for sequencing with illumina MiSeq. But there is a problem. PCR fragments cluster very poorly. There are very less no. of sequences that...
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