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Forum: Vendor Forum 07-15-2015, 09:58 AM
Replies: 2
Views: 2,413
Posted By jpmirandam
Dear cknox, I bought mate pair kits, both...

Dear cknox,

I bought mate pair kits, both for illumina and ion torrent in order to prepare 8 kb insert libraries.

None of the protocols has worked for us and we lost more than 98% of the DNA...
Forum: Core Facilities 06-09-2015, 07:43 AM
Replies: 0
Views: 3,299
Posted By jpmirandam
OMICS-Solutions: Sequencing provider from Chile to South América

- We provide library prep, sequencing and bioinformatic services on:

GS-FLX+
GS-Junior+
Ion Torrent PGM
Illumina MiSeq
Illumina HiSeq (per lane service)

Why sequence whit us?
Forum: Ion Torrent 06-09-2015, 07:05 AM
Replies: 0
Views: 1,519
Posted By jpmirandam
Iont Truemate Barcoding?

Have you ever tried to put barcodes to a truemate library?

I want to run more than one sample per sequencing run and I don´t know if I can use common barcode adapters instead Barcode Adapter A.
...
Forum: Sample Prep / Library Generation 10-30-2014, 07:23 AM
Replies: 5
Views: 2,948
Posted By jpmirandam
I have the same problem. I make a change on the...

I have the same problem. I make a change on the fragmentation protocol whit covaris (from a 550 pb insert to a 400 pb insert) but the mean size was almost the same (900 bp aprox.). I think that could...
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