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Forum: Illumina/Solexa 02-05-2015, 10:33 AM
Replies: 8
Views: 3,048
Posted By RNA
I would highly recommend this blog from...

I would highly recommend this blog from CoreGenomics which tries to address this issue using the data published by the SEQC group last year:
...
Forum: Illumina/Solexa 02-04-2015, 02:20 PM
Replies: 8
Views: 3,048
Posted By RNA
"20X coverage" for RNA-Seq is difficult to define...

"20X coverage" for RNA-Seq is difficult to define since the copy number varies for transcripts across at least 4 orders of magnitude within a tissue. Therefore estimating "coverage" for RNA-Seq is...
Forum: Illumina/Solexa 01-14-2015, 02:40 PM
Replies: 3
Views: 3,739
Posted By RNA
Need a little more information to make a clear recommendation

Hi ildem,

Before we help you decide which kit is best, it would be nice to know more about the quality of the RNA you will be using in this study. Is it high quality RNA from fresh samples? Or is...
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