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Forum: General 01-26-2010, 02:21 AM
Replies: 9
Views: 2,669
Posted By 454Sequencing
Hi brjordan, The list of 625 publications...

Hi brjordan,

The list of 625 publications enabled by 454 Sequencing may be found here:
http://www.454.com/publications-and-resources/publications.asp

To clarify, the Publications section of...
Forum: 454 Pyrosequencing 01-05-2010, 05:52 AM
Replies: 15
Views: 8,908
Posted By 454Sequencing
Dear sulfobus, Yes, the emPCR kits that...

Dear sulfobus,

Yes, the emPCR kits that support GS FLX Titanium series sequencing of amplicons are available. You can find information on current products at our website, specifically at...
Forum: Bioinformatics 01-04-2010, 05:52 AM
Replies: 8
Views: 4,473
Posted By 454Sequencing
Hi jffkm, If you are a customer you can...

Hi jffkm,

If you are a customer you can obtain these documents directly from the my454 customer portal (http://www.454.com/my454). All of the user manuals as well as other documentation may be...
Forum: 454 Pyrosequencing 11-30-2009, 10:31 AM
Replies: 1
Views: 8,669
Posted By 454Sequencing
Hi Triticum, In fact, there is a certain...

Hi Triticum,

In fact, there is a certain percentage of droplets that contain more than one template fragment. We minimize the occurrence of this via appropriate library dilution. A good reason for...
Forum: Bioinformatics 11-30-2009, 05:11 AM
Replies: 8
Views: 4,473
Posted By 454Sequencing
Hi kmcarr, Yes, it is possible to do a full...

Hi kmcarr,

Yes, it is possible to do a full run of only A- or B- amplicon sequencing with a single Lib-A emPCR kit. However, the minimum emPCR enrichment required for each gasket format must be...
Forum: Bioinformatics 11-30-2009, 04:54 AM
Replies: 8
Views: 4,473
Posted By 454Sequencing
Hi SeqMonster, The Emulsion Oil cups as...

Hi SeqMonster,

The Emulsion Oil cups as provided in the kit are identical. However, the emPCR Reagent Kit (Lib-A) contains reagents for both A- and B- side amplicon sequencing. In other words,...
Forum: 454 Pyrosequencing 11-30-2009, 04:44 AM
Replies: 34
Views: 14,563
Posted By 454Sequencing
Dear Bruno, You're welcome! As we've...

Dear Bruno,

You're welcome!

As we've only recently released the new cDNA Rapid Library protocol I'm unaware of any studies concerning the use of the Evrogen products in combination with this...
Forum: 454 Pyrosequencing 11-25-2009, 01:59 AM
Replies: 13
Views: 6,077
Posted By 454Sequencing
Hi flxlex, One certainly "could" do that -...

Hi flxlex,

One certainly "could" do that - the world won't end, assuredly! However, it's not a recommended/supported strategy. Amplicons and shotgun reads have different analysis pipelines as they...
Forum: 454 Pyrosequencing 11-24-2009, 12:14 PM
Replies: 9
Views: 49,575
Posted By 454Sequencing
Hi seqAll, Unless I am mistaken you might be...

Hi seqAll,

Unless I am mistaken you might be confusing Raw Wells with quality filtered reads. Quality filtered reads are themselves a percentage of Raw Wells, since they represent the output of...
Forum: 454 Pyrosequencing 11-24-2009, 09:33 AM
Replies: 9
Views: 49,575
Posted By 454Sequencing
Hi Phillip and seqAll, My first...

Hi Phillip and seqAll,

My first recommendation would be to consult the 454 software manual for more information on what defines Raw Wells. The Manuals section of the my454 customer site...
Forum: 454 Pyrosequencing 11-20-2009, 11:14 AM
Replies: 34
Views: 14,563
Posted By 454Sequencing
Emanuel - Yes. The easiest path is to follow up...

Emanuel - Yes. The easiest path is to follow up with your local Roche representative to get access to our software. Be sure to send me a PM if you don't meet with success.

kmcarr - Yes, we have...
Forum: 454 Pyrosequencing 11-20-2009, 07:35 AM
Replies: 34
Views: 14,563
Posted By 454Sequencing
Hi Phillip, One clarification - the Rapid...

Hi Phillip,

One clarification - the Rapid Library protocol actually employs a single adaptor moiety, and no longer an A-B pair of adaptors. This is why there is no more ssDNA melt step and why...
Forum: 454 Pyrosequencing 11-20-2009, 05:05 AM
Replies: 2
Views: 2,151
Posted By 454Sequencing
Hi wjeck, This week 454 released a new...

Hi wjeck,

This week 454 released a new version of our mapper/assembler software that provides for structural variation detection functionality. Other new features include the ability of handling...
Forum: 454 Pyrosequencing 11-20-2009, 04:59 AM
Replies: 34
Views: 14,563
Posted By 454Sequencing
Hi everyone, If you've not heard, earlier...

Hi everyone,

If you've not heard, earlier this week we announced the availability of a new cDNA library preparation protocol. This is a straightforward, general utility transcriptome sequencing...
Forum: 454 Pyrosequencing 11-10-2009, 05:13 AM
Replies: 28
Views: 10,567
Posted By 454Sequencing
You're welcome, Susanne. The REM e System is a...

You're welcome, Susanne. The REM e System is a robotic module for automation of post-breaking steps - in other words, enrichment and sequencing primer annealing. It is a deck accessory intended for...
Forum: 454 Pyrosequencing 11-10-2009, 04:47 AM
Replies: 28
Views: 10,567
Posted By 454Sequencing
Hi everyone, Thanks for your interest in the...

Hi everyone,

Thanks for your interest in the REM e System. This unit is not presently available as it is slated for launch in early 2010. I'll post more information when it's available and in the...
Forum: Bioinformatics 11-09-2009, 04:15 AM
Replies: 8
Views: 4,473
Posted By 454Sequencing
Hi biohumin, You will find some useful...

Hi biohumin,

You will find some useful information in the following post:
http://seqanswers.com/forums/showpost.php?p=8662&postcount=9

-as well as in my earlier post in the same thread.

If...
Forum: 454 Pyrosequencing 11-06-2009, 01:19 PM
Replies: 9
Views: 4,269
Posted By 454Sequencing
Hi Cambridge454, Allow me to lend a hand....

Hi Cambridge454,

Allow me to lend a hand. Are you familiar with Technical Bulletin 011-2009? If not, it is available on the my454 customer site
(http://www.454.com/my454) under the "Technical...
Forum: Bioinformatics 10-30-2009, 11:15 AM
Replies: 1
Views: 2,825
Posted By 454Sequencing
Hi ankitgupta.iitg, If you've not heard...

Hi ankitgupta.iitg,

If you've not heard previously, 454 Life Sciences will shortly launch a new transcriptome assembler as part of our next product update. I think you will find this new tool...
Forum: 454 Pyrosequencing 10-29-2009, 06:19 AM
Replies: 11
Views: 6,496
Posted By 454Sequencing
Dear HMorrison, I think I can help clarify...

Dear HMorrison,

I think I can help clarify things. It is true that the upcoming GS FLX Titanium series Amplicon emPCR kits will contain all reagents needed for generating enriched beads for...
Forum: 454 Pyrosequencing 08-19-2009, 11:52 AM
Replies: 15
Views: 8,908
Posted By 454Sequencing
Hi everyone, Allow me to clear a few things...

Hi everyone,

Allow me to clear a few things up. 454 Life Sciences plans to release methods, reagents, and software for GS FLX Titanium series amplicon sequencing in the fall. Please stay tuned for...
Forum: 454 Pyrosequencing 04-21-2009, 12:46 PM
Replies: 0
Views: 1,502
Posted By 454Sequencing
Lightbulb @454Sequencing on twitter!

Hi, everyone!

Are you using Twitter (http://www.twitter.com)? If so, I'd like to pass along our new account to you: @454Sequencing (http://twitter.com/454Sequencing).

As we wrote in our...
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