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Forum: RNA Sequencing 04-04-2018, 03:37 AM
Replies: 7
Views: 2,607
Posted By AlexCalderwood
Please see attached for "per base sequence...

Please see attached for "per base sequence content" for one of the reverse read problem files post trimming. (Sorry, in a previous post I screwed up forward and reverse reads -> _1 is reverse,...
Forum: RNA Sequencing 04-03-2018, 01:31 AM
Replies: 7
Views: 2,607
Posted By AlexCalderwood
They were made using "NEB next ultra directional...

They were made using "NEB next ultra directional library kit", which uses dUTP method to retain strandedness, and should give an insert size of ~200bp
Forum: RNA Sequencing 03-30-2018, 11:36 AM
Replies: 7
Views: 2,607
Posted By AlexCalderwood
Hi Phillip, thanks for your attention - what...

Hi Phillip, thanks for your attention - what would you like to know about the libraries?

Yes exactly, the forward "_1 file" reads are red and orange lines in the thumbnail, the reverse reads are...
Forum: RNA Sequencing 03-28-2018, 07:15 AM
Replies: 7
Views: 2,607
Posted By AlexCalderwood
low GC% peak in one end of paired end reads

Hi,
I have paired end RNA seq data prepared from Brassica napus using TruSeq kit. After adapter trimming, FastQC shows a second low GC% peak per sequence in the _1.fq files. The _2 files all look...
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