Forum: Illumina/Solexa
03-19-2017, 02:04 PM
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Replies: 6
Views: 1,689
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Forum: Illumina/Solexa
05-09-2016, 12:43 PM
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Replies: 10
Views: 2,644
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Forum: Sample Prep / Library Generation
09-22-2015, 01:18 AM
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Replies: 2
Views: 1,216
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Forum: Sample Prep / Library Generation
09-21-2015, 07:13 AM
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Replies: 2
Views: 1,216
RNA Later - DNA Prep
Hi all,
Just wondering if anyone has ever used DNA isolated from cells that have been stored in RNA later for NGS, Metagenomics and methlylation analysis specifically?
I am assuming that the...
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Forum: Illumina/Solexa
07-11-2015, 12:44 AM
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Replies: 2
Views: 1,196
Sorry but I don't think this is possible, at...
Sorry but I don't think this is possible, at least not from any area that user can access. It may be possible for tech support to help but may have trouble getting to them at the weekend. However if...
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Forum: Sample Prep / Library Generation
06-18-2015, 02:49 PM
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Replies: 18
Views: 16,520
New tape station, 4200, to be released soon,...
New tape station, 4200, to be released soon, likely shipping in September. May be wise to wait and see what the offer is....
Looks like it will be capable of running 96 samples in "walk away" mode.
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Forum: Illumina/Solexa
06-15-2015, 03:43 AM
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Replies: 10
Views: 3,159
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Forum: General
05-17-2014, 01:04 AM
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Replies: 0
Views: 2,327
Identify live cells only
Hi all,
Would welcome opinions on the following. I am trying to identify viable bacteria in solution after antimicrobials treatment to distinguish what has been killed and what has not. Dead...
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Forum: Sample Prep / Library Generation
04-07-2014, 01:36 PM
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Replies: 5
Views: 3,970
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Forum: Sample Prep / Library Generation
01-28-2014, 12:46 PM
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Replies: 10
Views: 6,203
Silly question first. Have you run them on the...
Silly question first. Have you run them on the chip a second time? It almost looks like bleed through from the ladder to be honest. Adapter concatamers is also possible but we have done a lot of chip...
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Forum: Illumina/Solexa
09-10-2013, 12:56 AM
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Replies: 19
Views: 6,942
True the primers are pretty long (I think about...
True the primers are pretty long (I think about £20 each) and can be expensive but I guess you need to weigh up the benefits of a one step library prep over the longer protocol. If you have not seen...
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Forum: Illumina/Solexa
09-09-2013, 03:00 PM
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Replies: 19
Views: 6,942
If you are thinking about gene specific pcr I...
If you are thinking about gene specific pcr I would include the illumina adapters and barcodes in the primers so you olmy need one pcr to create the libraries must like meta genomic preps. You will...
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Forum: Illumina/Solexa
09-03-2013, 04:22 PM
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Replies: 8
Views: 7,390
See if you can get access to a fluidigm access...
See if you can get access to a fluidigm access array. You might be able to prep all of the samples on a single chip on the access array in a much quicker time than by hand.
You could otherwise try...
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Forum: Illumina/Solexa
07-16-2013, 06:16 AM
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Replies: 24
Views: 12,929
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Forum: Illumina/Solexa
06-19-2013, 12:30 PM
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Replies: 4
Views: 1,743
Bear the following in mind however if you are...
Bear the following in mind however if you are performing a de novo on a miseq. Basespace/miseq reporter only samples 500Mb from each sample and tries to perform the assembly on this. If you have...
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Forum: Illumina/Solexa
06-03-2013, 01:24 PM
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Replies: 30
Views: 26,780
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Forum: Sample Prep / Library Generation
06-03-2013, 12:37 PM
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Replies: 10
Views: 2,191
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Forum: Sample Prep / Library Generation
06-03-2013, 12:34 PM
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Replies: 2
Views: 2,174
Easiest thing to do would be to dilute all your...
Easiest thing to do would be to dilute all your samples to a standard concentration and then pool equal amount accordingly. I have done this with 96 sample plenty of times. It takes time to do it but...
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Forum: Illumina/Solexa
06-03-2013, 12:23 PM
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Replies: 30
Views: 26,780
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Forum: Sample Prep / Library Generation
05-22-2013, 05:46 AM
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Replies: 3
Views: 2,917
Haloplex libraries
I have just completed a run of a FC using the Haloplex exome kit. While the protocol is nice and easy the cluster numbers are confusing me a bit. We quantified initially using the bioanalyzer and...
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Forum: Illumina/Solexa
03-10-2012, 01:46 AM
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Replies: 3
Views: 2,543
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Forum: Sanger/Dye Terminator
09-27-2011, 12:29 PM
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Replies: 5
Views: 4,821
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Forum: RNA Sequencing
10-13-2010, 01:44 AM
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Replies: 2
Views: 1,988
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Forum: General
10-12-2010, 04:41 AM
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Replies: 0
Views: 1,892
Sequencing small DNA fragments (25bp)
Hi all,
Just wondering if anyone has any experience of the following. I want to sequence a single fragment of RNA to confirm it is what I think it is. Any suggestions on how to go about it. The...
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Forum: RNA Sequencing
10-12-2010, 04:24 AM
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Replies: 2
Views: 1,988
Sequencing RNA
Hi all,
Just wondering if anyone has any experience of the following. I want to sequence a single fragment of RNA to confirm it is what I think it is. Any suggestions on how to go about it. The...
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