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Forum: Illumina/Solexa 10-10-2019, 01:11 AM
Replies: 2
Views: 1,208
Posted By seq198
For PCR-free libs kapa quant kit makes sense, as...

For PCR-free libs kapa quant kit makes sense, as qubit will not deliver accurate results. he different buffers for diluting should not have a big effect for what I can say. 5 times pipetting up and...
Forum: Sample Prep / Library Generation 04-25-2018, 05:15 AM
Replies: 9
Views: 5,970
Posted By seq198
You could consider enzymatic fragmentation...

You could consider enzymatic fragmentation (cheaper and faster than Covaris). Nearly all NGS kit provider have an option for enzymatic fragmentation now. The FS kit from NEB is promising in our...
Forum: Sample Prep / Library Generation 04-25-2018, 05:04 AM
Replies: 3
Views: 3,402
Posted By seq198
You may also try to use less primers in your...

You may also try to use less primers in your index PCR
Forum: Sample Prep / Library Generation 05-22-2017, 01:55 AM
Replies: 68
Views: 25,183
Posted By seq198
Thank you for the information and congrats for...

Thank you for the information and congrats for finding a company for commercialisation! Do you know whether the custom sequencing primer does also work when CATS libs are multiplexed with other libs,...
Forum: Sample Prep / Library Generation 09-28-2016, 03:57 AM
Replies: 6
Views: 3,432
Posted By seq198
Have you been able to find a solution? We are...

Have you been able to find a solution? We are seeing also high levels of smaller peaks.
Forum: Sample Prep / Library Generation 04-14-2016, 01:40 AM
Replies: 282
Views: 142,489
Posted By seq198
Thank you! I had a look at the manual. What...

Thank you! I had a look at the manual. What disturbed me was the combination of 5´blocked ISPCR primers with Thruplex. I fear that this might cause a loss of 3´and 5´terminal sequences in the lib...
Forum: Sample Prep / Library Generation 04-13-2016, 11:44 PM
Replies: 282
Views: 142,489
Posted By seq198
This thread is indeed very interesting, and I...

This thread is indeed very interesting, and I read through all comments. As I have only limited experience with nextera, I wanted to ask whether the system is also compatible with other lib prep...
Forum: Sample Prep / Library Generation 04-13-2016, 03:40 AM
Replies: 68
Views: 25,183
Posted By seq198
Maybe I missed it, but is paired-end sequencing...

Maybe I missed it, but is paired-end sequencing possible using CATS? Especially for longer ssDNA fragments?
Forum: RNA Sequencing 12-21-2015, 04:44 AM
Replies: 4
Views: 2,124
Posted By seq198
We are following exactly NEB kit instructions...

We are following exactly NEB kit instructions (NEBNext® Ultra™ RNA Library Prep Kit for Illumina® and NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina), we start with 500 ng of total...
Forum: RNA Sequencing 12-07-2015, 04:11 AM
Replies: 4
Views: 2,124
Posted By seq198
Hedgehog shaped cDNA library

Hi there,

I would like to ask whether someone has ever experienced a similar problem with hexamer primed cDNA libraries?

We have prepared a cDNA library starting with high quality RNA from mice...
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