SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 8 of 8
Search took 0.00 seconds.
Search: Posts Made By: AvantS
Forum: Sample Prep / Library Generation 05-29-2019, 09:00 AM
Replies: 12
Views: 1,148
Posted By AvantS
That sounds very likely to me as well. My team...

That sounds very likely to me as well. My team and I will redo the pipeline keeping those suggestions in mind. Thank you.
Forum: Sample Prep / Library Generation 05-28-2019, 08:40 AM
Replies: 12
Views: 1,148
Posted By AvantS
Thank you for the great advice. I'll run a gel...

Thank you for the great advice. I'll run a gel confirmation to see if my adapter ligated libraries still have sample.
Forum: General 05-26-2019, 08:42 PM
Replies: 6
Views: 1,123
Posted By AvantS
This...

This (https://www.beckman.com/reagents/genomic/cleanup-and-size-selection/pcr/a63880) is the product we use.

The 1.2X is volume ratio of bead to sample. For example, if I wanted to purify 100uL...
Forum: Sample Prep / Library Generation 05-24-2019, 11:32 AM
Replies: 12
Views: 1,148
Posted By AvantS
Our post-amp cleanup used 0.8x bead. The samples...

Our post-amp cleanup used 0.8x bead. The samples will be diluted to 20nM for sequencing.

It's not possible that the elution buffer was used instead of EtOH because each U-bottom plate used for...
Forum: General 05-24-2019, 09:57 AM
Replies: 6
Views: 1,123
Posted By AvantS
We use AMPure beads at 1.2X and a 96-well bead...

We use AMPure beads at 1.2X and a 96-well bead extractor protocol (http://www.vp-scientific.com/Magnetic_Bead_Separation_Extractor.php) for our PCR purification. This lets us purify all 96 wells with...
Forum: Sample Prep / Library Generation 05-24-2019, 09:39 AM
Replies: 12
Views: 1,148
Posted By AvantS
@itstrieu The workflow includes 3 PCRs: End...

@itstrieu The workflow includes 3 PCRs: End repair & A-tailing PCR, Adapter Ligation, and an Indexing. Input concentration was 100ng/uL per sample. Quantification after cleanup was not done, since...
Forum: Sample Prep / Library Generation 05-23-2019, 03:45 PM
Replies: 12
Views: 1,148
Posted By AvantS
@SeqSuave. It was made on the same day as the...

@SeqSuave. It was made on the same day as the bead cleanup.
Forum: Sample Prep / Library Generation 05-23-2019, 10:41 AM
Replies: 12
Views: 1,148
Posted By AvantS
Loss of entire sample set after SPRI cleanup

Hello all,

I was prepping samples for WGS Using the Kapa HyperPrep workflow. After the final cleanup with 0.8x AMPure SPRI beads, my gel confirmation showed that most of my samples were lost.
...
Showing results 1 to 8 of 8

 


All times are GMT -8. The time now is 04:26 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO