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Forum: Ion Torrent 06-06-2018, 06:18 PM
Replies: 37
Views: 39,288
Posted By zany
I didn't get information that KAPA 2G could...

I didn't get information that KAPA 2G could tolerate uracil.

But this one is claimed capable:

KAPA HiFi HotStart Uracil+ ReadyMix (1 x 1.25 mL)
Forum: Ion Torrent 06-06-2018, 06:06 PM
Replies: 37
Views: 39,288
Posted By zany
I think you are right, when you use Endonuclease...

I think you are right, when you use Endonuclease VIII, you might need T4 pol(or DNA pol1).When you use Endonuclease IV, you might need T4 PNK.

Then the next question is: How to determine...
Forum: Ion Torrent 06-06-2018, 05:58 PM
Replies: 37
Views: 39,288
Posted By zany
Thank you all the above guys, you provided many...

Thank you all the above guys, you provided many informations.

I'm also trying to make my own home-made ampliseq libray kit (for Ion torrent and Illumina)

Based on posts above, I think that the...
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