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Forum: Sample Prep / Library Generation 04-12-2017, 11:27 AM
Replies: 12
Views: 2,664
Posted By renuka22
Is it a good idea to do DNAse treatment because I...

Is it a good idea to do DNAse treatment because I got to know that the yield I will be getting from this tissue after clean up will be around 20ng/ul. I am planning to do a polyA isolation later and...
Forum: Sample Prep / Library Generation 04-12-2017, 09:06 AM
Replies: 12
Views: 2,664
Posted By renuka22
Ok. Thanks. Do you have any idea why are the Zymo...

Ok. Thanks. Do you have any idea why are the Zymo columns so cheaper than Qiagen. Which one is good? Zymo also has some clean up kits with DNAse in those. Do those work good?
Forum: Sample Prep / Library Generation 04-12-2017, 08:05 AM
Replies: 12
Views: 2,664
Posted By renuka22
Ok got you. I think it would be better to do a...

Ok got you. I think it would be better to do a clean up first and then measure concentration. Can the DNAse also be a source of degradation because I have not done the DNase treatment? I don't have...
Forum: Sample Prep / Library Generation 04-11-2017, 01:10 PM
Replies: 12
Views: 2,664
Posted By renuka22
Here is the nanodrop image..

Here is the nanodrop image..
Forum: Sample Prep / Library Generation 04-11-2017, 01:05 PM
Replies: 12
Views: 2,664
Posted By renuka22
Hi, I think you are absolutely right. There...

Hi,

I think you are absolutely right. There is a peak at 270nm seen on nanodrop. Is there any way I can clean up the samples with a kit. Will that help? What can be a good solution to this...
Forum: Sample Prep / Library Generation 04-11-2017, 09:13 AM
Replies: 12
Views: 2,664
Posted By renuka22
Hi, Sorry about the missing information. I...

Hi,

Sorry about the missing information. I checked on nanodrop and it showed concentration in the range of 200-300 ng/ul. I am working on rat primary retinal ganglion cells and isolating polysomal...
Forum: Sample Prep / Library Generation 04-10-2017, 12:15 PM
Replies: 12
Views: 2,664
Posted By renuka22
RNA degradation?

Hello,

PFA the electropherogram image of RNA degradation on bioanalyzer. There were no peaks from samples which I can conclude as no RNA or RNA has degraded completely. But the RIN value is 3.4....
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