Forum: General
02-23-2021, 02:21 PM
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Replies: 2
Views: 215
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Forum: Oxford Nanopore
11-30-2020, 09:45 AM
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Replies: 1
Views: 1,214
No remodeling needed.
All Nextera libraries...
No remodeling needed.
All Nextera libraries are PCR amplified to be sequenceable. After the PCR amplification they are wonderfully double-stranded throughout and should be blunt-ended (if a...
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Forum: Sample Prep / Library Generation
10-01-2020, 11:09 AM
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Replies: 3
Views: 1,242
You might want to have a look at this FAQ: ...
You might want to have a look at this FAQ:
https://dnatech.genomecenter.ucdavis.edu/faqs/my-pcr-free-libraries-do-not-look-as-expected-on-the-bioanalyzer-how-should-i-qc-pcr-free-libraries/
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Forum: Sample Prep / Library Generation
10-01-2020, 10:57 AM
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Replies: 5
Views: 1,600
Depends on the dye used and the amount of...
Depends on the dye used and the amount of primers. Ethidiumbrmide is ten times less sensitive for ssDNA compared to dsDNA. The sampe seems to be true for the Bioanalyzer, but such an instrument is...
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Forum: Sample Prep / Library Generation
09-26-2020, 07:39 AM
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Replies: 5
Views: 1,600
You could run aliquots of the reaction (before...
You could run aliquots of the reaction (before and after bead cleanups) on an agarose gel to see what is going on.
If the ligation does not work porperly, perhaps your samples have some chemical...
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Forum: Sample Prep / Library Generation
09-18-2020, 12:26 AM
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Replies: 5
Views: 1,801
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Forum: Sample Prep / Library Generation
09-14-2020, 11:23 PM
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Replies: 5
Views: 1,801
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Forum: General
08-05-2020, 02:30 PM
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Replies: 1
Views: 2,133
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Forum: Illumina/Solexa
08-02-2020, 06:18 PM
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Replies: 3
Views: 1,247
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Forum: Illumina/Solexa
08-01-2020, 05:59 PM
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Replies: 3
Views: 1,247
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Forum: Sample Prep / Library Generation
07-25-2020, 08:17 PM
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Replies: 1
Views: 1,642
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Forum: Oxford Nanopore
07-24-2020, 12:53 PM
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Replies: 7
Views: 3,500
In our experience the ligation and associated...
In our experience the ligation and associated reagents is the most sensitive part of library preps. I would expect other reagents to last longer past the expiration date, but I won't make any...
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Forum: Illumina/Solexa
07-23-2020, 06:15 PM
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Replies: 1
Views: 1,448
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Forum: Introductions
07-23-2020, 06:09 PM
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Replies: 1
Views: 1,443
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Forum: Oxford Nanopore
07-22-2020, 02:26 PM
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Replies: 7
Views: 3,500
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Forum: Bioinformatics
06-18-2020, 09:18 PM
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Replies: 1
Views: 1,399
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Forum: Sample Prep / Library Generation
06-05-2020, 06:20 PM
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Replies: 1
Views: 1,988
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Forum: Illumina/Solexa
05-13-2020, 01:27 PM
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Replies: 2
Views: 2,041
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Forum: Introductions
04-28-2020, 01:55 PM
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Replies: 5
Views: 2,400
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Forum: Illumina/Solexa
04-24-2020, 09:26 PM
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Replies: 2
Views: 1,961
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Forum: Sample Prep / Library Generation
04-16-2020, 04:20 PM
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Replies: 6
Views: 1,946
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Forum: Bioinformatics
04-14-2020, 10:57 PM
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Replies: 1
Views: 807
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Forum: Sample Prep / Library Generation
03-31-2020, 07:50 PM
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Replies: 4
Views: 2,421
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Forum: Illumina/Solexa
03-05-2020, 04:40 PM
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Replies: 2
Views: 1,420
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Forum: Sample Prep / Library Generation
02-06-2020, 09:22 PM
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Replies: 1
Views: 1,509
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