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Forum: Sample Prep / Library Generation 01-05-2018, 03:12 PM
Replies: 5
Views: 1,735
Posted By omans
The double peak looks like degraded RNA. So there...

The double peak looks like degraded RNA. So there must be something that is causing the single primary cells, but not pooled sample or single jurkats to degrade. If you can identify that factor, you...
Forum: Sample Prep / Library Generation 01-05-2018, 01:40 PM
Replies: 5
Views: 1,735
Posted By omans
Primary lymphocytes have a significantly lower...

Primary lymphocytes have a significantly lower level of mRNA than cultured cells.

I've found that scRNA-seq is very sensitive to sample handling. Are you using fresh or frozen T cells? How long...
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